摘要
对利用聚乙二醇(polyethylene glycol,PEG)-磷酸盐双水相体系从Pseudoalteromonas arctic GS230发酵液中直接萃取分离低温α-淀粉酶进行研究。探讨PEG分子量、相浓度、pH值对低温α-淀粉酶在PEG-磷酸盐双水相中分配系数、纯化系数及回收率的影响,并进行直线放大实验。结果表明:在pH5.0,PEG-100015%-磷酸盐15%的双水相体系中,低温α-淀粉酶的纯化系数及回收率分别为4.8和87%。
An aqueous two-phase system consisting of polyethylene glycol and phosphate was applied to extract cold-adapted α -amylase from fermentation culture supernatant of Pseudoalteromonas arctic GS230. The effects of phase composition, molecular weight of PEG and pH on enzyme partitioning and selective enrichment were studied, and the extraction procedure established was verified by linear scale-up experiments. A maximum purification fold of 4.8 and enzyme activity recovery of 87% were obtained when an aqueous 15% PEG-15% phosphate system at pH 5.0 was used for the extraction.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第18期159-162,共4页
Food Science
基金
连云港市科技局资助项目(CG0714)
关键词
双水相体系
分离
低温α-淀粉酶
aqueous two-phase system （ATPS）：extraction
cold-adapted α-amylase
