摘要
应用mRNA差异显示技术,对高耐SO2玉米自交系Q9进行了SO2胁迫下的基因差异表达分析。结果显示,30对引物组合进行的PCR扩增中,获得了13条差异表达的cDNA片段,其中3条诱导表达,2条增强表达,6条减量表达,2条抑制表达。序列分析和数据库比对表明,GenBank中只搜寻到5条cDNA序列,其中2条增强表达的cDNA片段D1和D5分别与氨基酸结合蛋白ABP、锌指结构转录因子蛋白DOF部分序列高度同源,其他诱导表达的3条cDNA(D3、D6、D11)功能未知,可能是新的cDNA片段。经半定量RT-PCR分析验证,D1和D5转录水平受SO2胁迫表达显著增强,推测D1和D5可能参与了玉米对SO2胁迫的抗性反应。
Expression of differential genes under SO2 stress was studied in SO2-tolerant maize inbred Q9 by mRNA differential display technique.The results showed that 13 differential cDNA fragments were amplified from 30 pairs of anchor primers and random primers combinations,among which expression of 3 cDNAs were induced,2 cDNAs were up-regulated,6 cDNAs were down-regulated and 2 cDNAs were inhibited under SO2 stress.Sequence analysis and homology alignment showed that only 5 cDNAs were found in GenBank,in which the up-regulated expression cDNA D1 had 82% similarity with an amino acid-binding protein（ABP） and the up-regulated expression cDNA D5 had 91% similarity with a DNA binding protein with a zinc finger（DOF）,and other 3 cDNA fragments might be unknown new genes.Semi-quantitative RT-PCR analysis demonstrated that the expression of D1 and D5 were up-regulated significantly by SO2 stress in tolerant inbred Q9,indicating that the two differential cDNAs D1 and D5 may be involved in resistant responses of maize plants to SO2 stress.
出处
《西北植物学报》
CAS
CSCD
北大核心
2009年第9期1736-1741,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金项目(30971548)
教育部科学技术研究重点项目(208080)
