摘要
〔目的〕了解上海口岸2001—2005年艾滋病病毒I型感染者(HIV-1)毒株基因亚型分布、变异情况及流行特征。〔方法〕收集2001—2005年上海口岸51份HIV抗体检测确认阳性的血样标本,提取前病毒DNA,使用套式聚合酶链反应进行体外扩增,获得核心蛋白(gag)和包膜蛋白(env)区基因的核酸片段,测定其序列和进行亚型分析。〔结果〕37份标本扩增阳性,共检出8个亚型,包括B亚型11份;CRF01-AE10份;CRF02-AG5份;A亚型4份;C亚型4份;G亚型、B’,和CRF07_BC亚型各1份。上海口岸毒株亚型分布于五大洲15个国家和地区,主要流行CRF01-AE和B亚型,从2004年开始出现其它亚型逐渐增多趋势。〔结论〕上海口岸流行的HIV-1毒株复杂多样,出入境人员是我国HIV毒株输入的主要渠道,流行毒株以性接触传播为主,AE重组毒株具有较强的传播优势。
Objective To study the distribution of human immunodeficiency virus-1（HIV-1）strains subtypes at Shanghai frontier port from 2001 to 2005, and to understand its epidemiology feature. Method Proviral DNA was extracted from 51 HIV-1 positive blood samples, HIV env and gag gene were amplified by nested-PCR using specific primer pairs and sequenced to identify subtypes. Result A total of 37 sequences were amplified from 51 collected blood samples, 8 kinds of subtypes were found：ll cases of B, 10 cases of CRF-01AE, 5 cases of CRF02-AG,4 cases of A, 4 cases of C and 1 cases of G, B＇, CRF07_BC, respectively. These HIV infected were coming from 15 different countries and regions of 5 continents. Subtype CRF01-AE and B appeared to be the major epidemic strain of HIV-1 at Shanghai frontier port and there were other subtypes rising trend from 2004. Conclusion There are plenty kinds of HIV subtypes among Entry-Exit passengers who are the main source of HIV strain imported. Meanwhile, sexual contact is the dominant route for HIV spreading and subtype CRFO1-AE appeared to have advantages in transmission.
出处
《中国国境卫生检疫杂志》
CAS
2009年第5期339-345,共7页
Chinese Journal of Frontier Health and Quarantine
关键词
人类免疫缺陷病毒1型
亚型
序列分析
分子
流行病学
Human immunodeficiency virus type 1
Subtype
Sequence analysis
Molecular
Epidemiology
