摘要
目的：探讨与修订银黄颗粒的质量标准。方法：应用HPLC法,选用Betasil C18色谱柱（4.6×200 mm,5μm）;流动相为甲醇-水-冰醋酸（50∶50∶1）;流速1.0 ml/min;检测波长274 nm;理论板数按黄芩苷峰计不少于1500,对银黄颗粒中的黄芩苷进行含量测定。结果：黄芩苷进样量在0.208~1.04μg范围内与峰面积成良好的线性关系（r=0.9999,n=5）;黄芩苷的回收率为99.94%,RSD=0.92%。结论：本方法可对银黄颗粒的主要药味进行准确的定量测定,能更为有效的控制银黄颗粒的质量。
Objective： To explore and revise the quality standards of Yinhuang Granule.Methods： HPLC and Betasil C18 column（4.6×200 mm,5 μm）were used;the mobile phase was methanol-water-glacial acetic acid（50∶50∶1） with flow rate of 1.0 ml/min and the detection wave length of 274 nm;the peak number of theoretical plate was no less than 1500 pairs of baicalin and the content of baicalin in Yinhuang Granule was detected.Results： The sample size of baicalin in the 0.208~1.04 μg had a good linear relationship（r=0.9999,n=5） with the peak area within the framernrk;the recovery rate of baicalin wae 99.94% ,RSD=0.92%,Conclusion;This method can be an accurate quantitative measurement for major constituents of Yinhuang Granule ,thus effiectively controlling over the quality of Yinhuang Granule.
出处
《河南中医》
2009年第10期979-981,共3页
Henan Traditional Chinese Medicine
关键词
银黄颗粒
质量标准
高效液相色谱法
Yinhuang Granule
quality standards
high-performance liquid chromatography
