摘要
本实验应用碱性凝胶电泳技术探讨了活性氧自由基在紫外线照射人体皮肤形成环丁烷嘧啶二聚体中的作用。人皮肤ＤＮＡ加抗氧化剂后，经紫外线照射，Ｔ４Ｎ５切割，电泳，最后进行凝胶成像系统分析。结果可见，抗氧化剂对环丁烷嘧啶二聚体的形成起到一定的抑制作用，尤其是β胡萝卜素和超氧化物歧化酶（ＳＯＤ）比谷胱甘胱（ＧＳＨ）和过氧化氢酶（ＣＡＴ）的作用明显，但均未能完全阻止ＣＰＤ的形成，这提示活性氧自由基在ＣＰＤ的形成中起部分的介导作用。
To define further the role of reactive oxygen species (ROS) in ultraviolet radiation (UV) induced the formation of Human skin's CPD, experiments were performed using alkaline gel electrophoresis technique. Purified DNA was incubated with antioxidants. After DNA was exposed to UV, T4 Endonuclease V was added, electrophoresis was taken, CPD was measured by Gel Imaging and Image Analysis. It was found that antioxidants exhibited some inhibitory effects on the formation of CPD, β carotene and superoxide dismutase (SOD) are more obvious than GSH and CAT, but none of which were able to block the formation completely. The results suggest that the formation of CPD is only partly dependent on ROS.
出处
《癌变．畸变．突变》
CAS
CSCD
1998年第6期331-335,共5页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金
