摘要
目的:探讨紫草素对佐剂性关节炎大鼠滑膜炎症的影响。方法:SD大鼠分为4组(每组10只):正常对照组,佐剂性关节炎组,甲氨蝶呤治疗组,紫草素治疗组。测量大鼠后肢关节肿胀程度,实验第二十一天杀死大鼠,分离右后肢关节滑膜,应用免疫组织化学方法结合病理学图像分析系统,研究滑膜组织中单核-巨噬细胞(CCR5阳性)的浸润情况。结果:甲氨蝶呤和紫草素均能减轻关节肿胀(P<0.05)。甲氨蝶呤治疗组和紫草素治疗组关节滑膜CCR5阳性细胞明显减少,且紫草素治疗组更低。结论:紫草素减轻大鼠佐剂性关节炎的炎症反应,减轻关节肿胀和减少滑膜组织炎症细胞浸润,为紫草素治疗类风湿关节炎(RA)提供了实验依据。
Objective：To explore the effects of shikonin on synovitis of adjuvant arthritis （AA） rats.Methods：SD rats were divided into four groups （n=10 each） ： normal control group,AA model control group,methotrexate （MTX） treatment group and shikonin treatment group.Then the swelling of the rats＇ hind limbs was evaluated. After the rats were killed on 21 d,monocyte-macrophage （CCR5 positive） infiltration in the right hind-limb synovium was studied using immunohistochemical technique and image analysis.Results ：The swelling of AA rats treated by MTX and shikonin was relieved （P〈0.05）. The monocyte-macrophage infiltration was lower in the treatment group （P〈 0.05）, but it is lower （P〈0.O5）in shikonin treatment group compared with MTX treatment group.Conclusion：The effects of shikonin on AA rats include：decreasing manifestations of arthritis, alleviating joint swelling and reducing inflammatory infiltration of synovial tissue.Our study might provide an experimental basis for the treatment of rheumatoid arthritis with shikonin.
出处
《现代医药卫生》
2009年第19期2891-2893,共3页
Journal of Modern Medicine & Health
基金
郧阳医学院药护学院学生科研基金资助(NO.200801)
