摘要
试验旨在探究新疆驴冷冻精液的最佳稀释保护液与处理方法。以假阴道采集新疆驴精液,并用5种不同稀释保护液稀释冷冻后,选取其中较为理想的稀释液作为对照组,再通过分别添加4%、5%、6%、8%甘油处理试验,最后再选用添加甘油后冷冻效果最优的稀释液为对照来进行离心浓缩试验。结果表明:①5号稀释液冷冻—解冻精液后,其活力、顶体完整率要显著高于其他4种稀释液(P<0.05);②以5号稀释液为对照组,添加6%甘油(7号稀释液)冷冻后,精子的活力、顶体完整率要显著高于添加4%、5%、8%甘油组(P<0.05);③以7号稀释液为对照组,通过离心浓缩,精液在冷冻后其活力和精子顶体完整率都显著提高(P<0.05)。结果提示,将驴精液用6%葡萄糖、2%乳糖、6%甘油为主的稀释液稀释,并经500 r/min离心浓缩10 min处理后,其冷冻效果最好。
This experiment was conducted to study the best extender and the way on frozen semen of Xinjiang donkey. The Xinjiang donkey semen was collected by artificial vagina, and diluted with five different extenders. Then the better extender was selected as a control group, and was respectively added by 4 %, 5 %, 6 %, 8 % glycerol. Finally the best extender was selected as a control group, and was concentrated. The results showed that： （1）The frozen-thawing semen motility and acrosomal integrity that was frozen by the 5th extender were significantly higher（P〈0.05） than that of 1, 2, 3, 4; （2）Add 6 percent glycerol on the 7th extender was better（P〈0.05） than the addition of 4%, 5%, 8%; （3）The frozen-thawing semen motility and acrosomal integrity that was frozen by the 7th concentrated extender were significantly higher（P〈0.05） than others. The resuits indicated that it was useful that the semen was diluted with the 6% glucose, 2 % sucrose, 6%glycerol extender, and was concentrated 10 minutes in 500 r/min before being frozen.
出处
《中国畜牧兽医》
CAS
北大核心
2009年第10期107-110,共4页
China Animal Husbandry & Veterinary Medicine
基金
新疆生产建设兵团塔里木畜牧科技重点实验室项目(HS20701)
关键词
驴精液
冷冻
甘油
离心
donkey semen
frozen
glycerol
centrifugation
