摘要
目的观察不同浓度的丙泊酚对体外培养的兔气管黏膜上皮细胞纤毛运动的影响,探讨一氧化氮(NO)在丙泊酚影响纤毛运动中的作用。方法无菌条件下从健康新西兰兔获取离体气管标本,分离上皮组织培养7～8d后观察。以Hank平衡盐溶液(HBSS)将丙泊酚的终浓度分别稀释为1、10、20、50、100、200μg/ml。将组织块随机分为8组,前7组分别加入HBSS(P0)和上述6种浓度的丙泊酚(P1、P2、P3、P4、P5、P6),第8组(P7)在加入0.1mmol/L的一氧化氮合酶(NOS)总抑制剂——L-NMMA后再以200μg/ml丙泊酚处理。在加药前(T0),加药后1min(T1)、5min(T2)、10min(T3)、20min(T4)、30min(T5),采用高速数码摄像系统采集纤毛摆动图像,通过IPLabV3.65软件分析,计算纤毛摆动频率(ciliarybeatfrequency,CBF),分别进行组内和组间对比。结果①P0组、P1组和P2组的CBF值在各时点与各自的基础值比较均无明显变化;P1组和P2组的CBF值与P0组相应时间点相比无明显变化;②P3组CBF在T1时点比基础值和P0组T1时点值明显升高(P<0.05);在随后的各个观察时点与基础值及P0组相应时间点比较无明显变化;③P4、P5、P6组加药后各个观察时点的CBF均明显高于基础值和P0组各时点值(P<0.05);④P7组加药后各时点的CBF值与基础值相比均无明显变化。结论50μg/ml以上浓度的丙泊酚可促进纤毛摆动,50、100、200μg/ml浓度的丙泊酚可能通过促进NO合成和释放而加速离体纤毛运动。
Objective To investigate the effects of different concentration of propofol on tracheal cilia movement. Methods Ciliary beat frequency （CBF） of cultured rabbit bronchial epithelial cells was measured by high-speed digital microscopy before （T0, baseline） and 1min （T1）, 5 min （T2）, 10 min （T3）, 20 min （T4）, 30 min （T5） after the specimen was dipped into HBSS （P0） or propofol solution at the concentration of 1,10,20,50,100,200 μg /ml （P1～P6） and 200 μg/ml propofol with 0.1μmol L-NMMA （P7） respectively. Results CBF of cultured bronchial epithelial cells exposed to HBSS showed no significant changes within 30 minutes. In 1 and 10 μg/ml propofol solution, CBF showed no significant changes within 30 minutes. In 20 μg/ml propofol solution, CBF increased significantly and reached the peak in one minute. Then it decreased gradually and showed no significance in 5 ～30 minutes. In propofol solution above 50 μg/ml, CBF increased significantly and reached the peak in one minute, and maintained the high level in the following observing period. CBF of the specimen pretreated with 0.1μmol L-NMMA showed no significant changes. Conclusions 1～200 μg/ml propofol does not inhibit CBF in cultured rabbit tracheal epithelial cell. Under certain concentrations （above 50μg/ml）, propofol may increase CBF. Propofol might stimulate ciliary motility via promoting synthesis and release of NO.
出处
《北京医学》
CAS
2009年第10期609-611,共3页
Beijing Medical Journal
关键词
丙泊酚
气管黏膜
上皮细胞
纤毛
Propofol Tracheal mucosa Epithelial cells Cilia
