摘要
将组织型纤溶酶原激活剂（ｔ－ＰＡ）基因ｃＤＮＡ经多次亚克隆插入到真核表达载体ｐＳＶＬ的ＳＶ４０启动子和ｐｃＤＮＡ３的ＣＭＶ启动子下游，构建了重组表达质粒ｐＳＶＬ－ｔＰＡ和ｐｃＤＮＡ３－ｔＰＡ经酶切和Ｓｏｕｔｈｅｒｎ杂交鉴定，ｔ－ＰＡ基因插向正确，可用于哺乳动物细胞和个体表达系统中表达。
Two expressing plasmids,pSVL-tPA and pcDNA3-tPA,containing the tissue-type plasminogen activator (t-PA) cDNA gene,were constructed by inserting the t-PA cDNA into the downstream of the SV40 promoter and the CMV promoter,respectively.Restriction enzymes digestion and southern bolt demonstrated that t-PA gene had been inserted into right site.The recombinant plasmids could be used as expressing vectors in mammalian cells.
出处
《河南农业大学学报》
CAS
CSCD
1998年第4期335-338,344,共5页
Journal of Henan Agricultural University
