摘要
以大叶山杨的超级实生苗的当年生枝条、成年优树根部萌条当年生嫩枝和成年优树树冠当年生嫩枝切段做外植体,采用连续继代培养和微型嫁接法进行幼化处理技术研究。结果表明：随连续继代次数的增加及微嫁接后再继代次数的增加,茎条的幼化程度随之提高,徽扦插生根率也在提高。成年优树根部萌条当年生嫩茎连续继代4～5次,微扦插生根率可达100%;成年优树树冠当年生嫩茎连续继代6～7次,微扦插生根率可达100%;用组培苗作砧木微嫁接后再进行微繁,连续继代3～4次,微扦插生根率可达100%。
We conducted the rejuvenation of Populus davidiana vat. macrophylla with explants, i.e. branches from seedlings, root sprouting and crown of mature healthy trees, by continuous subculture and micrografting technologies. The results showed： the degree of rejuvenation of braches positively increased with the generation number of the continuous subculture, and the generation number of continuous subculture after mierografting, which also improved the root promoting rate. To reach 100% root promoting rate of micrografting, it took 4 ～ 5 generations of continuous subculture of root sprouting from mature healthy tree, and 6 ～ 7 generations of continuous subculture for the branches from crown of mature healthy trees. The root promoting rate was up to 100% after 3 - 4 generations of continuous subculture when the mierografting conducted on the tissue cultural seedlings as stocks.
出处
《吉林林业科技》
2009年第5期18-20,44,共4页
Journal of Jilin Forestry Science and Technology
关键词
大叶山杨
幼化处理
连续继代
微型嫁接
Populus davidiana var.macrophylla
rejuvenation
continuous subculture
micrografting
