摘要
目的观察Graves病患者外周血辅助性T细胞(Th细胞)表面CCR5和CD30的表达,探讨Th1/Th2细胞失衡在Graves病患者发病中的作用。方法选择89例Graves病患者,将其分为Graves病未治疗组(Graves病组,31例)、Graves病甲巯咪唑治疗组(甲巯咪唑组,30例)和Graves病13Ⅰ1治疗组(13Ⅰ1组,28例),另选择35例健康者作为对照组。采用流式细胞技术检测4组血清甲状腺相关指标及外周血淋巴细胞中CCR5及CD30的表达量,以此作为反映Th1/Th2免疫应答强度的指标。结果Graves病组患者CD30表达量高于对照组,CCR5/CD30低于对照组,差异有统计学意义(P<0.01);甲巯咪唑组和13Ⅰ1组患者CCR5/CD30高于Graves病组,差异有统计学意义(P<0.05),但与对照组比较差异无统计学意义(P>0.05)。相关分析显示,CD30表达量与促甲状腺素受体抗体(TRAb)水平呈正相关(r=0.38,P<0.01),CCR5/CD30与TRAb水平呈负相关(r=-0.33,P<0.01),CCR5水平与TRAb水平无相关性(r=-0.14,P>0.05)。结论Graves病患者Th细胞表面CD30表达明显增加,CCR5/CD30比值降低,提示Th1/Th2细胞失衡参与了Graves病的免疫调节过程;而甲巯咪唑和13Ⅰ1治疗能缓解Graves病患者Th1/Th2细胞失衡。
Objective To observe the expressions of CCR5 and CD30 on peripheral blood helper T cells and Th1/Th2 cell disbalance in pathogenesis of Graves.Methods Eighty-nine Graves patients were divided into Graves′group（n=31,untreated）,thiamazole group（n=30）and 131Ⅰ group（n=28）,and 35 healthy subjects were enrolled as control.The indexes related to thyoid and the expression of CCR5 and CD30 in peripheral blood lymphocytes were detected by flow cytometry,which was taken as the index reflecting intensity of Th1/Th2 immune response.Results CD30 expression was higher and CCR5/CD30 was lower in Graves′ group than in control,the difference was significant（P〈0.01）.CCR5/CD30 was higher in thiamazile group and 131Ⅰ groups than in Graves′ group,the difference was significant（P〈0.05）.Related analyses showed that CD30 expression was positively correlated with thyrotropin receptors（r=0.38,P〈0.01）,CCR5/CD30 negatively with TRAb（r=-0.33,P〈0.01）,CCR5 not correlated with TRAb level（r=-0.14,P〉0.05）...更多.Conclusion In Graves′ patients,CD30 expression on Th cells increases markedly,CCR5/CD30 ratio decreases,suggesting that Th1/Th2 cell imbalance takes part in immuno-regulation process of Graves disease.Thiamazole and 131Ⅰ can improve Th1/Th2 cell imbalance.
出处
《中国全科医学》
CAS
CSCD
北大核心
2009年第20期1854-1856,共3页
Chinese General Practice
