妊娠期肝内胆汁淤积症患者胎盘组织HIF-1α、HIF-2α mRNA的表达及其临床意义被引量：1

The mRNA expression of HIF-1αmRNA and HIF-2αmRNA in the placenta of intrahepatic cholestasis of pregnancy and their clinical significance

下载PDF

导出

摘要目的探讨缺氧诱导因子-1α(HIF-1α)mRNA、缺氧诱导因子-2α(HIF-2α)mRNA在妊娠期肝内胆汁淤积症(ICP)患者胎盘组织上的表达及其与胎儿缺氧的关系。方法采用实时荧光定量法分别检测轻度ICP组、重度ICP组、正常妊娠组三组胎盘组织上的HIF-1αmRNA、HIF-2αmRNA的表达。结果35例胎盘组织均表达HIF-1α、HIF-2αmRNA,重度ICP组HIF-1αmRNA表达水平显著低于轻度组和正常组(P<0.05),且其表达与ICP分度之间存在等级相关(r=-0.409,P=0.015),轻度组与正常组差异无统计学意义(P>0.05)。HIF-2αmRNA表达水平三组间差异无统计学意义(P>0.05),其表达与ICP分度之间无等级相关性(r=-0.263,P=0.463)。结论ICP患者HIF-1αmRNA表达明显降低是机体适应慢性缺氧的表现,当在宫缩等急性缺氧应激下发生失代偿时可能是导致胎儿不良结局的原因。HIF-1αmRNA可作为判断病情程度的一个指标。 Objective To study the mRNA expression of hypoxia inducible factor-1α（HIF-1α） mRNA and hypoxia inducible factor-2α（HIF-2α） in the placenta of intrahepatic cholestasis of pregnancy and to find the relationship of their expressions and fetal anoxia. Methods HIF-1α mRNA and HIF-2α mRNA were measured by real-time fluorescent quantitation （RTQ -PCR） in the placenta tissues of the mild ICP pregnancy women, severe ICP pregnancy women and normal pregnancy women. Results HIF-1α, HIF-2α mRNA were expressed in all 35 placenta tissues. The expression in the placenta tissue of severe ICP pregnancy women was significant lower than that of the mild ICP pregnancy women and normal pregnancy women （ P 〈 0.05 ）. There was significant correlation between the expression level and ICP scale division（r=-0.409 ,P = 0.015）. There was no significant difference between the mild ICP pregnancy women and normal pregnancy women（ P 〉 0.05） the expressions of HIF-2α mRNA in the placenta tissue was no significant difference among the three groups （P 〉 0.05）, and there was no rank correlation between the expression level and ICP scale division（r=-0.263, P=0.463）, Conclusion The lower expression of HIF-1α mRNA of ICP pregnancy women was a manifest of organism adaptation to chronic anoxia. The expression of HIF -1α mRNA is one of important indexes to judge the pathogenetic conditions of ICP pregnancy women.

作者倪观太李志芳

机构地区皖南医学院弋矶山医院妇产科安庆市立医院

出处《安徽医学》 2009年第10期1138-1140,共3页 Anhui Medical Journal

基金安徽省教育厅基金(KJ2008B322)

关键词缺氧诱导因子-1Α 缺氧诱导因子-2Α 实时荧光定量法妊娠期肝内胆汁淤积症胎盘 Hypoxia inducible factor-1α（HIF-1α） Hypoxia inducible factor-2α（HIF-2α） Real -time fluorescent quantitation Intrahepatic cholestasis of pregnancy Placenta

分类号 R714.2 [医药卫生—妇产科学]

引文网络
相关文献

参考文献11

1漆洪波,邵勇,吴味辛,张建华.妊娠肝内胆汁淤积症分度诊断和处理的临床意义[J].中华妇产科杂志,2004,39(1):14-17. 被引量：151
2Semenza GL. Targeting HIF - 1 for cancer therapy. Nat Rev Cancer,2003, 3 (10) :721 - 732.
3Rajakumar A, Conrad K. Expression, ontogeny, and regulation of hypoxia - inducible transcription factors in the human placenta. Biol Reprod ,2000,63:559 - 569.
4胡雅毅,刘淑芸,王晓东,姚强.妊娠肝内胆汁淤积症患者胎盘组织缺氧及复氧时缺氧诱导因子1α表达水平的变化[J].中华妇产科杂志,2007,42(1):54-56. 被引量：6
5Yu AY, Frid MG, Larissa A, et al. Temporal, spatial, and oxygen - regulated expression of hypoxia - inducible factor - 1 in the lung. Am J Physiol Lung Cell Mol Physiol, 1998,275 : L818 - L826.
6刘伯宁陈忠年.晚期胎盘病理.妇产科病理学[M].上海:上海医科大学出版社,1996.320.
7Gradin K, McGuire J, Wenger RH, et al. Functional interference between hypoxia and dioxin signal trans duction pathways: competition for recruitment of the ARNT transcription factor. Mol Cell Biol, 1996,16(10) ;5221 - 5231.
8姚元庆,David Barlow,Ian Sargent.缺氧诱导因子1和缺氧诱导因子2在人类着床前胚胎的表达[J].生殖医学杂志,2006,15(4):227-233. 被引量：1
9Kallio PJ, Pongratz I,Gradin K,et al. Activation of hypoxia inducible factor 1 alpha: posttranscriptional regulation and conformational change by recruitment of the Arnt transcription factor. Proc Natl Acad Sci USA, 1997,94( 11 ) :5667 - 5672.
10夏松云,陈竹钦,李力.妊娠肝内胆汁淤积症患者高水平胆汁酸对血管内皮细胞生长因子的作用[J].中华妇产科杂志,2002,37(11):669-671. 被引量：23

二级参考文献45

1吴维光,姚元庆,李东红,于月成,范小斌,张伟.缺氧诱导因子1α在妊高征和正常妊娠胎盘组织中的差异表达[J].细胞与分子免疫学杂志,2005,21(3):362-364. 被引量：11
2范小斌,姚元庆,李东红,张伟,尹国武,李巧红.HIF-2α对绒毛组织缺氧时VEGF表达的作用及其意义[J].中华围产医学杂志,2005,8(3):154-156. 被引量：1
3吴味辛熊昌碧.妊娠肝内胆汁淤积－附134例临床分析[J].中华妇产科杂志,1984,19:17-17.
4刘伯宁，中华妇产科杂志，1988年，23卷，9页
5戴钟英，上海医学，1986年，9卷，440页
6戴钟英.妊娠肝内胆汁淤积症[A].曹泽毅主编.中华妇产科学[C].北京:人民卫生出版社,1999.500～507.
7Fischer B,Bavister BD.Oxygen tension in the oviduct and uterus of rhesus monkeys,hamsters and rabbits[J].J Reprod Fertil,1993,99 (2):673-679.
8Semenza GL.Targeting HIF-1 for cancer therapy[J].Nat Rev Cancer,2003,3(10):721-732.
9Fukuda R,Hirota K,Fan F,et al.Insulin-like growth factor 1 induces hypoxia-inducible factor 1-mediated vascular endothelial growth factor expression,which is dependent on MAP kinase and phosphatidylinositol 3-kinase signaling in colon cancer cells[J].J Biol Chem,2002,277(41):38205-38211.
10Laughner E,Taghavi P,Chiles K,et al.HER2 (neu) signaling increases the rate of hypoxia-inducible factor 1 alpha (HIF-1 alpha) synthesis:novel mechanism for HIF-1-mediated vascular endothelial growth factor expression[J].Mol Cell Biol,2001,21 (12):3995-4004.