摘要
建立HPLC法测定苦参中苦参碱和氧化苦参碱含量。色谱柱采用Aichrom NH_2(250 × 4.6mm,5 μ m),流动相为乙腈-乙醇-3%磷酸溶液(80:10:10),流速:1.0mL/min,检测波长:210nm,进样量为10μL,柱温:30℃。苦参碱在0.5～10 μ g/mL范围内有良好的线性关系(r=0.9998),氧化苦参碱在0.5～10 μ g/mL范围内有良好的线性关系(r=0.9997)。苦参碱平均加标回收率为100.42%(n=5),RSD为1.91%;氧化苦参碱平均加标回收率为100.26%(n=5),RSD为2.03%。该方法操作简单、重现性好、检测灵敏度、准确度高,且样品分离效果好。本方法适用于苦参药材中苦参碱和氧化苦参碱的含量测定。
A high performance liquid chromatography （HPLC） method was established to determine the contents of matrine and oxymatrine in Sophora flavescens Ait. Aichrom NH2 （250× 4.6 mm, 5 μm）, acetonitrile -ethanol -3 %phosphoric acid （80 ： 10 ： 10） as the mobile phase, with a detection wavelength at 210 nm. The flow rate was 1.0 mL/min. The injection volume was 10μL, and the column temperature was set at 30 ℃. The calibration curves of matrine and oxymatrine were in good linearity over the rang of 0.5-10μg/mL （r=0.9998） and 0.5-10 μg/mL （ r= 0.9997）. The average recovery were 100.42%, 100.26%, and RSD were 1.91%, 2.03% （n = 5） for matrine and oxymatrine. The method is simple, and accurate, and can be used for the determination of matrine and oxymatrine in Sophora flavescens Ait.
出处
《现代仪器》
2009年第5期43-45,共3页
Modern Instruments
