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肝癌患者外周血AFP mRNA检测的临床追踪观察 被引量:2

Detection of Hepatocellular Carcinoma Cells in Peripheral Blood of Patients with HCC
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摘要 采用逆转录及巢式聚合酶链式反应(RTPCR)和Southern杂交方法对肝癌患者的早期血行微转移灶进行检测并做临床随访观察。10例原发性肝癌患者(HCC),经手术切除的癌组织,检测AFPmRNA均为阳性。39例HCC患者中有24例外周血检测为阳性,阳性率61.5%。1a时间随访到28例患者,9例阴性结果的病人,1例死亡,死亡率为11.0%。3例临床证实有转移灶。19例阳性结果的病人,9例死亡,死亡率为47.3%,4例临床证实有转移灶。RTPCR结果不同的两组经卡方检验,P<0.01,患者预后也有明显的差异。检测HCC患者外周血中癌细胞是阳性结果,可以说明癌细胞已进入血循环。随访结果显示HCC患者外周血中AFPmRNA检测呈阳性则同患者出现血行微转移灶及预后有一定的相关性,可以给临床提供一个肿瘤有远处转移的参考指标。 In order to searching an early indicator of hematogenous spreading metastasis of HCC,AFP mRNA was detected by RT PCR and Southen blot in cancer tissues and peripheral blood of patients with HCC,the clinical observation was carried out in 28 cases for 1 year.The results showed that AFP mRNA was found in all of 10 HCC tissues and in 24/39 cases (61.5%) of peripheral blood .During the one year,one patient died (11%) and 3 were found clinically metastasis in 9 AFP mRNA negative cases,nine died (47.3%) and 4 got metastasis in 19 positive cases.In comparison with the two groups,there was singnificant difference in prognosis( P <0.01).It suggests that AFP mRNA in peripheral blood of HCC may be an indicator of circulating HCC cells and associated with HCC hematogenous spreading metastasis.
出处 《实用癌症杂志》 1998年第4期257-259,共3页 The Practical Journal of Cancer
关键词 原发性 肝细胞癌 SOUTHERN杂交 甲胎球蛋白 mRNA Hepatocellular carcinoma (HCC) Southern hybridization α Fetoprotein (AFP)
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  • 1Bleicher R J,Allegra DP,Nora DT,et al.Radiofrequency ablation in 447 complex unresectable liver tumors:lessons learned[J].Ann Surg Oncol,2003,10(1):52 -58.
  • 2Izumi N,Asahina Y,Noguchi O,et al.Rist factors for distant recurrence of hepatocellular carcinoma in the liver after complete coagulation by microwave or radiofrequency ablation[J].Cancer,2001,91(8):949 -956.
  • 3Curley SA,Izzo F,Ellis LM,et al.Radiofrequency ablation of hepatocellular cancer in 110 patients with cirrhosis[J].Ann Surg,2000,232(4):381 -391.
  • 4Buscarini L,Buscarini E.Therapy of HCC-radiofrequency ablation[J].Hepatogastroenterology,2001,48 (1):15-19.
  • 5Poon RT,Ng KK,Lam CM,e t al.Radiofrequency ablation for subcapsular hepatocellular carcinoma[J].Ann Surg Oncol,2004,11(3):281-289.
  • 6Seki T,Tamai T,Ikeda K,et al.Rapid progression of hepatocellular carcinoma after transcatheter arterial chemoembolization and percutaneous radiofrequency ablation in the primary tumour region[J].Eur Gastroenterol Hepatol,2001,13(3):291 -294.
  • 7Fontana RJ,Hamidullah H,Nghiem H,et al.Percutaneous radiofrequency thermal ablation of hepatocellular carcinoma:a safe and effective bridge to liver transplantation[J].Liver Transpl,2002,8(5):1165 -1174.
  • 8Mutsuko M,Naoshi N.Postoperative detection of a-fetoprotein mRNA in blood as a predictor for metastatic recurrence of hepatocellnlar carcinoma[J].Gastroenterol Hepatol,2001,16(4):445 -451.
  • 9Kedda MA,Kew MC,Skelton M,et al.Nonspecific messenger RNA of a-fetoprotein in peripheral blood in detecting early spread of hepatocellular carcinoma in black Africans[J].Gastroenterol Hepatol,1998,13 (6):885-891.
  • 10Kienle P,Weitz J,Klaes R,et al.Detection of isolated disseminated tumor cells in bone marrow and blood samples of patients with hepatocellular carcinoma[J].Arch Surg,2000,135(2):213 -218.

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