摘要
提取西太平洋"暖池"区深海沉积物样品总DNA,利用异化型亚硫酸盐还原酶(DSR)和甲基辅酶M还原酶(MCR)基因的特异引物,采用PCR-RFLP方法对沉积物样品中这两类功能基因多样性进行研究。该沉积物中的dsrAB基因分别来源于δ-变形菌中的6个属,其中最多的是脱硫弧菌属和脱硫杆状菌属;mcrA基因均来源于产甲烷古菌,其中主要是甲烷微菌。这些基因在系统发育树上都处于相对独立的分支。此外,还有较多的dsrAB,mcrA基因来源于未知的新属或新种。这些结果表明该海区沉积物中由微生物参与的硫和甲烷循环比较活跃,而且其中可能存在多种新的代谢途径。
The diversities of genes specific for dissimilatory sulfite reductase （DSR） and methyl-coenzyme M reductase MCR in metagenomic DNA of deep sediment of the West Pacific ＂warm pool＂ were studied by PCR-RFLP using primers specific for dsrAB and mcrA, respectively. The results showed that the dsrAB in the sample were come from 6 genus of δ-Proteobacteria, mainly Desulfovibrio and Desulfobacter. All ofthe mcrA in the sample were come from archaeal methanogens, mainly Methanomicrobia. These genes were tend to clustered within independent clades on phylogenetic tree respectively. Furthermore, many dsrAB and mcrA genes showed high similarity to those from unknown or new species. These results indicated that the microbial metabolisms, which may include some new metabolic pathway, were active in the S and CH4 cycles in deep sediment of ＂warm pool＂.
出处
《海洋学报》
CAS
CSCD
北大核心
2009年第4期78-86,共9页
基金
中国大洋协会项目(DYXM-115-02-2-04)
国家海洋局海洋生物遗传资源重点实验室基金(HY200502)资助
