摘要
运用基因、DNA、染色体、细胞和整个动物为终点的远期危害测试系统,对各类机动车(汽油车、柴油车、助动车)排出尾气的有机提取物的致突变性及潜在致癌性进行了评估,同时运用流行病学方法对接触人群进行调查。结果表明:(1)柴油车、助动车的颗粒有机提取物包含100多种化学物。(2)Ames、UDS、微核及SHE细胞恶性转化试验为阳性结果,具有致突变性及致癌性。(3)它们能使人胚细胞形成转化灶并发现其中的c-myc、p21及bcl-2表达增强。(4)动物吸人试验表明肺部受损且免疫功能受到抑制。(5)职业接触人群的呼吸道刺激症状及COPD发生率增加。
Using a series of assay standing for difference gentoxity end points, such as gene, DNA, chromosome, cells and animals to assess the risk of toxicity and harmful effect of various vehicle exhaust particle extract, especially the mutagenicity and potential carcinogenicity. The results as follows: (1) There are more than 100 composites in the organic extract of exhaust particles, some of them are mutagenical or have potential carcinogenicity; (2) They all showed positive effects in Ames assay, UDS, micronucleus assay and SHE cell malignantly transformed assay and strong mutagenicity; (3) Those extracts could make the KMB-13 cells to form transformed foci, in which the oncogene protein expression, such as c-myc, p21, bcl-2 were higher than ever; (4) after inhaling the exhaust, animal's pulmonary and immune function were be damaged; (5) The symptoms of respiratory tract and the risk of COPD (chronic obstructive pulmonary diseases) are rised in the occupational exposed workers.
出处
《上海环境科学》
CAS
CSCD
1998年第10期16-18,共3页
Shanghai Environmental Sciences
基金
卫生部
国家教委博士点科研项目
编号0768202
国家自然科学基金项目
编号39270576
关键词
机动车
尾气
致突变性
人体健康
大气污染
Vehicle Exhaust Mutagenicity Respiratory tract damage Immune damage
