摘要
目的检测生长抑素(SST)受体亚型SSTR1～3在胃癌组织中表达与分布,探讨其表达与胃癌临床病理特点的关系。方法选取手术切除35例胃癌组织标本和35例胃良性组织,采用免疫组织化学方法检测SSTR1、SSTR3(二步法)SSTR2(三步法)的表达,染色后按着色细胞百分数进行评分判定,观察SSTR1～3在胃癌组织中表达与分布,并比较SSTR亚型表达与胃癌临床病理特点的关系。结果这些抗体能特异检测到胃癌组织和胃良性组织受体的表达,35例胃癌组织中,SSTR1、SSTR2、SSTR3表达阳性率分别为80.0%(28/35),65.7%(23/35)与51.4%(18/35);35例胃良性组织表达阳性率为91.4%(32/35),88.6%(31/35)与80.0%(28/35),χ2分析表明,SSTR2、SSTR3在胃癌与胃良性组织表达差异有统计学意义(P<0.05),SSTR1在胃癌组织中的表达有降低,但无统计学差异。胃癌SSTR表达阳性率与患者性别、年龄,肿瘤大小、组织学分化类型、淋巴结转移、浆膜侵润以及肿瘤分期差异无统计学意义(P>0.05),但发现SSTR3与组织学分化类型相关(P=0.028)。结论胃癌组织和胃良性组织中有多种SSTR亚型表达,SSTR1、2亚型表达和肿瘤组织学分化类型及肿瘤分期无明显相关,SSTR3与组织学分化类型相关。应用SSTR特异型抗体做免疫组化可作为临床常规检测分析患者SSTR表达状况,并利于选用特异生长抑素类似物进行治疗。
Objective To detect the expression of somatostatin receptor subtypes SSTR1-3 in the of gastric carcinoma and to discuss the relationship between the expressions and characteristics of the tumor.Methods Immunohistochemical staining of somatostatin receptor subtypes was performed by the standard streptavidin-peroxidase（SP） or two-step technique in the paraffin sections of 35 gastric carcinomas and 35 normal gastric tissues.The expressions were scored according to the percentage of pigmenting cells.The relationship between the expression rate of somatostatin receptor subtypes and the clinicopathological parameters was analyzed and their presence,cellular,localization,distribution,expression pattern in 35 gastric carcinomas were analyzed.Results The antibodies sperifically detected the receptors in gastric carcinomas and normal gastric tissues.The expression rate of somatostatin receptor of subtype1,subtype 2 and subtype 3 in the specimens of gastric carcinoma were 80.0%（28/35）,65.7%（23/35） and 51.4%（18/35） respectively.The expression rates of 35 normal gastric tissue were 91.4%（32/35）,88.6%（31/35） and 80.0%（28/35）,there were significant differences of SSTR2,SSTR3 expressions between the two groups（P〈0.05）,SSTR1 expressions in gastric carcinomas had a decreased tendency,but there were no significant differences of SSTR1 expressions between the two groups.No difference of SSTR1-2 expression rate among sex,age,tumor size,differential degree,lymphonode metastasis,depth of invasion and UICC staging was found,but the expressions of SSTR3 were related to the differential degree（P=0.028）.Conclusion SSTR1,SSTR2,SSTR3 have been detected in gastric carcinoma.The expression rates of SSTR1,SSTR2 do not correlate with the differential degree and UICC staging of the tumor,the expression of SSTR3 is related to the differential degree.The immunohistochemistry with subtype specific antibodies can be used in clinical routine work to analyze SSTR expression patterns for each patient before treatment and to facilitate well directed individual medical therapy by administering subtype specific somatostatin analogues.
出处
《肿瘤基础与临床》
2009年第4期297-301,共5页
journal of basic and clinical oncology
关键词
受体
生长抑素
胃肿瘤
免疫组织化
receptors
somatostatin
stomach neoplasms
immunohistochemistry
