摘要
目的:建立反相高效液相法同时测定伤科接骨片中士的宁和马钱子碱的含量测定方法。方法:采用Waters Symmetry ODS C18(4.6mm×200mm,5μm)色谱柱;流动相:乙腈-磷酸三乙胺水溶液(11:89)[1.7ml磷酸、1.8ml三乙胺加水至1000ml];检测波长:254nm;流速:1.0ml/min;柱温:40℃。结果:士的宁在0.062～0.434μg范围内与峰面积呈良好的线性关系(r=0.9996),平均回收率为98.12%,RSD=0.73%(n=6);马钱子碱在0.051～0.357μg范围内与峰面积呈良好的线性关系(r=0.9998),平均回收率为96.54%,RSD=0.70%(n=6)。结论:本方法简便、准确、重现性好,可用于伤科接骨片中士的宁和马钱子碱的质量控制。
Objective： To establish a method for the content determination of strychnine and brucine in Shangkejiegu Tablets by RP-HPLC. Methods： The Waters Symmetry ODS C18 （4.6 mm×200 mm, 5 μm） column was used. The mobile phases consisted of acetonitrile, phosphoric acid and triethylamine solution（11：89） [1.7ml phosphoric acid and 1.8 ml triethylamine was diluted to 1 000 ml by water], the flow rate was 1.0 ml/min and the detection wavelength was at 254 nm. The column temperature was 40℃. Results： The linear ranges of strychnine was at 0.062-0.434μg （r=0.999 6）, the average recovery was 98.12% with a RSD of 0.73% （n=6）; the linear ranges of brueine was at 0.051-0.357μg （r=0.999 8）, the average recovery was 96.54% with a RSD of 0.70%（n=6）.Condusion： The method is fast, reliable, accurate and can be used in the quality control of strychnine and brucine in Shangkejiegu Tablets.
出处
《中国医药导报》
CAS
2009年第31期38-40,共3页
China Medical Herald
