摘要
目的探讨Ras相关核蛋白(Ran)在大鼠体细胞中的定位,及其与细胞周期相关蛋白间的相互作用。进一步揭示细胞周期调控和有丝分裂的分子机制。方法用组织块培养法成功地分离培养了大鼠皮肤成纤维细胞,并采用激光扫描共焦显微技术研究细胞有丝分裂过程中Ran的定位变化,至少重复3次。结果发现在有丝分裂间期,大鼠成纤维细胞中Ran主要分布于细胞核内,核仁内没有Ran分布,细胞质中Ran浓度极低。核膜破裂,细胞进入有丝分裂,Ran分布到整个细胞,但在染色体周围浓集,浓集区形态和纺锤体的形态一致;在后期和末期浓集区域随纺锤体的变化而变化,分布在被分开的染色体之间,其他地方浓度较低;核膜形成后又重新浓集于细胞核内;在整个有丝分裂中,Ran在细胞分裂区的细胞膜下未见特殊分布。结论Ran的分布随大鼠细胞周期的变化而改变,与其对间期细胞核的内输和外运,有丝分裂中正常纺锤体的组装和染色体的正确分配,以及细胞周期的正常的调控作用是一致的。
Objective To study the localization of the Ras-related nuclear protein Ran in rat somatic ceils, and to investigate its interaction with the protein associated to the cell cycle. To reveal the molecular mechanism of the cell cycle control and mitotic division. Methods Fibroblasts derived from rat skin were successfully cultured with a normal tissue culture method, and investigated the localitation and possible roles of Ran during the cell mitotic division by using laser scanning confocal microscopy, repeated three times at least. Results The results showed that Ran was localized mainly in the nucleus, except for the nucleolus, in the intermediate stage rat skin fibroblasts ceil. Its density was very low in the cytoplasm. After the nuclear membrane broke down in mitoehysis, Ran distributed throughout the cell, but concentrated to the mitotic spindle, the concentrated area was uniformity with the spindle; in anaphase and telephase, the concentrated area changed with the spindle, localized between the separated chromosome, but lower in other sites. After mitosis, Ran migrated into the forming nucleus. There was no special distribution for Ran under the membrane of the cell fission. Conclusion Ran has a ceU cycle-dependent localization and this is consistent with its regulatory roles in nuclear import and export in rat intermediate ceils, the spindle assembly correctly, the correct chromosome segregation, and the cell cycle regulation.
出处
《解剖学报》
CAS
CSCD
北大核心
2009年第5期785-788,共4页
Acta Anatomica Sinica
基金
东北农业大学博士基金资助项目
