摘要
AIM:To investigate the effect of Chai-Qin-Cheng-Qi Decoction(CQCQD)on cefotaxime(CTX)concentration in pancreas of rats with acute necrotizing pancreatitis (ANP). METHODS:Sixty healthy male Sprague-Dawley rats were divided randomly into an ANP group(ANP model +CTX,n=20),treatment group(ANP model+CTX +CQCQD,n=20)and control group(normal rats+ CTX,n=20).ANP models were induced by retrograde intraductal injection of 3.5%sodium taurocholate (1 mL/kg),and the control group was injected intraductally with normal saline.All rats were injected introperitoneally with 0.42 g/kg CTX(at 12-h intervals for a continuous 72 h)at 6 h after intraductal injection. Meanwhile,the treatment group received CQCQD (20 mL/kg)intragastrically at 8-h intervals,and the ANP and control group were treated intragastrically with normal saline.At 15 min after the last CTX injection,blood and pancreas samples were collected for the determination of CTX concentration using validated high-performance liquid chromatography. Pathological changes and wet-to-dry-weight(W/D) ratio of pancreatic tissue were examined. RESULTS:Serum CTX concentrations in three groups were not significantly different.Pancreatic CTXconcentration and penetration ratio were lower in ANP group vs control group(4.4±0.6μg/mL vs 18.6± 1.7μg/mL,P=0.000;5%vs 19%,P=0.000),but significantly higher in treatment group vs ANP group (6.4±1.7μg/mL vs 4.4±0.6μg/mL,P=0.020;7% vs 5%,P=0.048).The histological scores and W/D ratio were significantly decreased in treatment group vs ANP and control group. CONCLUSION:CQCQD might have a promotive effect on CTX concentration in pancreatic tissues of rats with ANP.
AIM： To investigate the effect of Chai-Qin-Cheng-Qi Decoction （CQCQD） on cefotaxime （CTX） concentration in pancreas of rats with acute necrotizing pancreatitis （ANP）. METHODS： Sixty healthy male Sprague-Dawley rats were divided randomly into an ANP group （ANP model ＋ CTX, n = 20）, treatment group （ANP model ＋ CTX ＋ CQCQD, n = 20） and control group （normal rats ＋ CTX, n = 20）. ANP models were induced by retrograde intraductal injection of 3.5% sodium taurocholate （1 mL/kg）, and the control group was injected intraductally with normal saline. All rats were injected introperitoneally with 0.42 g/kg CTX （at 12-h intervals for a continuous 72 h） at 6 h after intraductal injection. Meanwhile, the treatment group received CQCQD （20 mL/kg） intragastrically at 8-h intervals, and the ANP and control group were treated intragastrically with normal saline. At 15 min after the last CTX injection, blood and pancreas samples were collected for the determination of CTX concentration using validated high-performance liquid chromatography. Pathological changes and wet-to-dry-weight （W/D） ratio of pancreatic tissue were examined. RESULTS： Serum CTX concentrations in three groups were not significantly different. Pancreatic CTX concentration and penetration ratio were lower in ANP group vs control group （4.4 ± 0.6 μg/mL vs 18.6±1.7μg/mL, P = 0.000; 5% vs 19%, P = 0.000）, but significantly higher in treatment group vs ANP group （6.4 ± 1.7 μg/mL vs 4.4 ± 0.6 μg/mL, P = 0.020; 7% vs 5%, P = 0.048）. The histological scores and W/D ratio were significantly decreased in treatment group vs ANP and control group. CONCLUSION： CQCQD might have a promotive effect on CTX concentration in pancreatic tissues of rats with ANP.
基金
Supported by National Key Technology R&D Program of China,No.2006BAI04A15
Major Diseases Fund of Sichuan Province Administration of Traditional Chinese Medicine,No.2007B03
关键词
头孢噻肟
胰腺炎
坏死性
急性
大鼠
Acute necrotizing pancreatitis
Cefotaxime
Chai Qin Cheng Qi Decoction
Drug penetration
Traditional Chinese medicine
