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重组腺病毒AdmIL-12对树突状细胞的基因转染 被引量：1

Gene Transfection of Dendritic Cells by Recombinant Adenovirus AdmIL-12
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摘要 目的:应用重组腺病毒AdmIL-12转染树突状细胞(DC)制备DC疫苗。方法:以重组腺病毒AdmIL-12转染DC后,用流式细胞仪检测DC的表型,用RT-PCR检测IL-12在DC内的表达,用EILSA法测定细胞培养上清液中的mIL-12和mIFN-γ的含量。通过IL-12刺激T细胞分泌IFN-γ的能力以检测DC分泌的IL-12的生物活性。结果:重组腺病毒AdmIL-12能高效转染DC,转染后DC的表型无显著改变,转染DC能大量分泌具有生物活性的IL-12。结论:腺病毒AdmIL-12成功转染DC,获得有效的DC疫苗。 Objective：To transfect the Dendritic cells （DC） with recombinant adenovirus vector AdmIL-12, make vaccine of DC. Methods：DC was transfected by AdmIL-12 and its phenotype was measured with floweytometery. The expression of IL-12 in DC was valued with PT-PCR method. The value of IL-12 and IFN-γ in culture supernatants was nanlyzed by ELISA. The biological activity of IL-12 secreted from DC was tested by its ability to stimulate T cells to secret IFN-γ. Results： Recombinant adenovirus AdmIL-12 transfected DC effectively. After transfetion, the phenotype of DC had no significant changement,IL-12 was expressed more in DC, DC secreted more IL -12 with biological activity. Conclusion： AdmlL-12 transfeeted DC effectively. The vaccine of DC was made successfully.
作者 邵长周 张浩茹 瞿介明 何礼贤 张艳红 王晋慧
机构地区 复旦大学附属中山医院 复旦大学附属华东医院 中国上海生命科学院生物细胞研究所
出处 《中国临床医学》 2009年第5期653-656,共4页 Chinese Journal of Clinical Medicine
基金 国家自然科学基金(30772019) 上海市重点学科建设项目(B115)
关键词 腺病毒 载体 树突状细胞 细胞因子 疫苗 Adenovirus Vector Dendritic cell Cytokine Vaccine
分类号 Q782 [生物学—分子生物学]
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共引文献22

同被引文献17

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中国临床医学
2009年 第5期

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