摘要
报道了以荧光素为指示剂，荧光法间接测定痕量ＳＣＮ－的新方法．在ｐＨ＝５．５～７．５的中性缓冲溶液中，Ｉ２与荧光素反应，使荧光素的荧光猝灭．当加入ＳＣＮ－时，ＳＣＮ－和Ｉ２反应使体系荧光增强，该体系激发波长、发射波长分别为４９４ｎｍ和５１５ｎｍ，ＳＣＮ－含量在１．０～６０μｇ／Ｌ范围内呈良好的线性关系，检测下限为１．０μｇ／Ｌ．该方法灵敏度高，选择性好，用于吸烟人和非吸烟人唾液中痕量ＳＣＮ－测定，结果令人满意．
A highly sensitive and selective fluorimetric new method has been developed for the determination of trace amounts of thiocyanate with fluorescein as the fluorogenic reagens (λ ex =494nm,λ em =515nm) at pH=5.5～7.5. In a neutral medium,I 2 reacts with fluorescein to produce a non fluorescence compound.If thiocynate are present,the fluorescence intensity of the solution increases with the increasing concentration of SCN - when approriate amounts of I 2 and fluorescein are added. The calibration range is linear for 1.0～60μg/L for thiocyanate and the detection limit is 1.0 μg/L, it has been applied to the determination of thiocyanate in the saliva with satisfactory results.
出处
《宁夏大学学报（自然科学版）》
CAS
1998年第3期250-252,共3页
Journal of Ningxia University(Natural Science Edition)
基金
宁夏区教委和区科委基金
