期刊文献+

三种临床血清酶国际、国内实验室比对结果分析及其参考方法的评估 被引量:1

Analysis of the international and domestic RELA results of three enzymes and evaluation of their reference methods
下载PDF
导出
摘要 目的分析2006年度至2007年度连续2年参加国际临床化学联合会(IFCC)的参考实验室丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)的室间质评(RELA)结果及卫生部临床检验标准委员会(以下简称卫生部)的酶学室间比对结果,评估其所用测定参考方法的检测质量及其完善程度。方法根据IFCC公布的ALT、AST、LDH的测定参考方法的标准操作程序(SOP)进行测定,根据《分析测量中不确定度的量化》(QUAM2000)计算合成标准不确定度,用日本临床检验标准协议会(JCCLS)旭化成株式会社认证酶标准物质评估参考方法的分析性能(准确度与不精密度)。结果2006年度的国际RELA与卫生部酶学室间比对结果室内变异系数(CV)均在1.5%~2.1%,2007年度的国际RELA结果与卫生部酶学室问比对结果室内CV均在1.1%以下,且本实验室与总体均值的偏倚整体低于2006年度,除2006年度卫生部酶学室间比对结果有3个值与总体均值的偏倚(7.99%,-7.02%,-13.97%)超过允许偏倚外,其余值均在允许偏倚范围内。ALT、AST、LDH标准物质室内CV均在1%以下,且均值偏倚靶值均低于1%。结论本实验室所用测定ALT、AST、LDH的参考方法已比较成熟稳定,2007年度的实验室比对结果优于2006年度。 Objective To analyze the results of three enzymes E alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH)] in the ring trials of Reference Laboratories In Medicine (RELA) organized by International Federation of Clinical Chemistry (IFCC) and the ring trials organized by National Center for Clinical Laboratory (NCCL) in 2006 and 2007, and evaluate the analytic characteristic and reliability of the reference methods of ALT,AST and LDH. Methods ALT, AST, and LDH were measured by the standard operating procedure (SOP) of reference methods published by IFCC, and the combined standard uncertainty was calculated according to Quantifying Uncertainty in Analytical Measurement (QUAM) 2000. The analytic characteristic of the reference methods was evaluated by human enzyme calibrators of Asahi Kasei Pharma corporation from Japanese Committee for Clinical Laboratory Standards (JCCLS). Results The within-laboratory coefficient of variation (CV) of the three enzymes was ranged from 1.5%-2.1% in the 2006 ring trials of RELA organized by IFCC and NCCL, while the within-laboratory CV of the three enzymes was less than 1.1% in the 2007 ring trials, and the bias(% ) was less than that in 2006. The values were within the deviation acceptance range except for the values of three samples(7.99% , -7.02%, -13.97% ). The within-laboratory CV of ALT, AST and LDH of the human enzyme calibrators of Asahi Kasei Pharma corporation were less than 1% , and the mean value deviation from target values was less than 1%. Conclusions The reference methods for the measurement of catalytic activity of ALT, AST and LDH have been improved and stable in our laboratory, and the results in the 2007 ring trials excelled those in the 2006 ring trials.
作者 欧元祝 居漪
出处 《检验医学》 CAS 北大核心 2009年第10期737-741,共5页 Laboratory Medicine
关键词 丙氨酸氨基转移酶 天门冬氨酸氨基转移酶 乳酸脱氢酶 参考方法 Alanine aminotransferase Aspartate aminotransferase Lactate dehydrogenase Reference method
  • 相关文献

参考文献8

  • 1Schumann G, Bonora R, Ceriotti F, et al. IFCC primary reference procedures for the measurement of catalytic activity concentrations of enzymes at 37 degrees C. Part 2. Reference procedure for the measurement of catalytic concentration of creatine kinase [J]. Clin Chem Lab Med, 2002, 40(6) :635-642.
  • 2Schumann G, Bonora R, Ceriotti F, et al. IFCC prinmry reference procedures for the measurement of catalytic activity concentrations of enzymes at 37 degrees C. Part 3. Reference procedure for the measurement of catalytic concentration of lactate dehydrogenase [J]. Clin Chem Lab Med, 2002, 40(6) :643-648.
  • 3Schumann G, Bonora R, Ceriotti F, et al. IFCC primary reference procedures for the measurement of catalytic activity concentrations of enzymes at 37 degrees C.International Federation of Clinical Chemistry and Laboratory Medicine. Part 4. Reference procedure for the measurement of catalytic concentration of alanine aminotrasferase[J]. Clin Chem Lab Med, 2002,40 (7) :718-724.
  • 4Schumann G, Bonora R, Ceriotti F, et al. IFCC primary reference procedures for the measurement of catalytic activity concentrations of enzymes at 37 degrees C.International Federation of Clinical Chemistry and Laboratory Medicine. Part 5. Reference procedure for the measurement of catalytic concentration of aspartate aminotransferase[J]. Clin Chem Lab Med, 2002,40 (7) :725-733.
  • 5Schumann G, Bonora R, Ceriotti F, et al. IFCC primary reference procedures for the measurement of catalytic activity concentrations of enzymes at 37 degrees C. International Federation of Clinical Chemistry and Laboratory Medicine. Part 6. Reference procedure for the measurement of catalytic concentration of gammaglutamyltransferase[J]. Clin Chem Lab Med, 2002, 40 (7) :734-738.
  • 6International Federation of Clinical Chemistry and Laboratory Medieine (IFCC) , Schumann G, Aoki R, et al. IFCC primary reference procedures for the measurement of catalytie activity concentrations of enzymes at 37 degrees C [J]. Clin Chem Lab Med, 2006,44 (9) :1146-1155.
  • 7EURACHEM/CITAC. Quantifying uncertainty in analytical measurement[S]. 2nd ed. 2000:4-31.
  • 8王涛,齐丽丽,刘海燕,田真,何振坤,李淑葵,但万春,徐国宾.六种临床诊断酶学测定参考方法的应用及国际比对结果分析[J].中华检验医学杂志,2008,31(3):264-269. 被引量:15

二级参考文献8

  • 1符颖操,罗茜.实验室间比对结果分析统计方法的探讨[J].理化检验(物理分册),2006,42(6):295-299. 被引量:55
  • 2徐国宾,吴南,王清涛.要重视血清酶学测定的标准化工作[J].临床检验杂志,2007,25(3):161-164. 被引量:25
  • 3Schumann G, Bonom R, Ceriotti F, et al. IFCC primary reference procedures for the measurement of catalytic activity concentrations of enzymes at 37 degrees C. Part 4.Reference procedure for the measurement of catalytic concentration of alanine aminotransferase. Clin Chem Lab Med, 2002, 40:718-724.
  • 4Schumann G, Bonora R, Ceriotti F, et al. IFCC primary reference procedures for the measurement of catalytic activity concentrations of enzymes at 37 degrees C. Part 2. Reference procedure for the measurement of catalytic concentration of creatine kinase. Clin Chem Lab Med, 2002, 40:635-642.
  • 5Schumann G, Bonora R, Ceriotti F, et al. IFCC primary reference procedures for the measurement of catalytic activity concentrations of enzymes at 37 degrees C. Part 3. Reference procedure for the measurement of catalytic concentration of lactate dehydrogenase. Clin Chem Lab Med, 2002, 40:643-648.
  • 6Schumann G, Aoki R, Ferrero C A, et al. IFCC primary reference procedures for the measurement of catalytic activity concentrations of enzymes at 37 degrees C. Part 8. Reference procedure for the measurement of catalytic concentration of alpha- amylase. Clin Chem Lab Med, 2006, 44-1146-1155.
  • 7Schumann G, Bonora R, Ceriotti F, et al. IFCC primary reference procedures for the measurement of catalytic activity concentrations of enzymes at 37 degrees C. Part 6. Reference procedure for the easurement of catalytic concentration of gammaglutamyltransferase. Clin Chem Lab Med, 2002, 40:734-738.
  • 8Schumann G, Bonora R, Ceriotti F, et al. IFCC primary reference procedures for the measurement of catalytic activity concentrations of enzymes at 37 degrees C. Part 5. Reference procedure for the measurement of catalytic concentration of aspartate aminotransferase. Clin Chem Lab Med, 2002, 40:725- 733.

共引文献14

同被引文献16

  • 1徐国宾,吴南,王清涛.要重视血清酶学测定的标准化工作[J].临床检验杂志,2007,25(3):161-164. 被引量:25
  • 2陈文祥,申子瑜,杨振华.临床检验中的测量不确定度[J].中华检验医学杂志,2007,30(9):967-971. 被引量:31
  • 3Schumann G, Bonora R, Ceriotti F, et al. IFCC primary referenceprocedures for the measurement of catalytic activity concentrationsof enzymes at 37 degrees C. Part 4. Reference procedure for themeasurement of catalytic concentration of alanine aminotransferase[J]. Clin Chem Lab Mad, 2002,40:718-724.
  • 4Schumann G, Bonora R, Ceriotti F, et al. IFCC primary referenceprocedures for the measurement of catalytic activity concentrationsof enzymes at 37 degrees C. Part 5. Reference procedure for themeasurement of catalytic concentration of aspartateaminotransferase[J]. Clin Chem Lab Med, 2002,40:725-733.
  • 5Schumann G, Bonora R, Ceriotti F, et al. IFCC primary referenceprocedures for the measurement of catalytic activity concentrationsof enzymes at 37 degrees C. Part 3. Reference procedure for themeasurement of catalytic concentration of lactate dehydregenase[J]. Clin Chem Lab Med, 2002, 40:643-648.
  • 6Schumann G, Bonora R, Ceriotti F, et al. IFCC primary referenceprocedures for the measurement of catalytic activity concentrationsof enzymes at 37 degrees C. Part 6. Reference procedure for themeasurement of catalytic concentration of gamma-glutamyltransferase[J]. Clin Chem Lab Med, 2002, 40:734-738.
  • 7Schumann G, Bonora R, Ceriotti F, et al. IFCC primary referenceprocedures for the measurement of catalytic activity concentrationsof enzymes at 37 degrees C. Part 2. Reference procedure for themeasurement of catalytic concentration of creatine kinase [J]. ClinChem Lab Med, 2002,40:635-642.
  • 8Schumann G, Aoki R,Ferrero CA, et al. IFCC primary referenceprocedures for the measurement of catalytic activity concentrationsof enzymes at 37 degrees C. Part 8. Reference procedure for themeasurement of catalytic concentration of alpha-amylase[J]. ClinChem lab Med, 2006,44:1146-1155.
  • 9Gerhard Schumann, Rainer Klaukl, Francesca Canalias, et al.IFCC primary reference procedures for the measurement of catalyticactivity concentrations of enzymes at 37 degrees C. Part 9.Reference procedure for the measurement of catalytic concentrationof alkaline phosphatase [J]. Clin Chem Lab Med, 2011, 49 :1439-1446.
  • 10EURACH EM/CITAC. Quantifying uncertainty in analyticalmeasurement[S]. 2nd ed. 2000:4-31.

引证文献1

二级引证文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部