摘要
番茄HP1和HP2是色素积累的负调控因子,在光形态建成和色素积累调控中起着重要作用.将番茄HP1、HP2基因片段导入到植物表达载体pBI121,用番茄果实特异表达的TMF7基因的启动子替换原有的CaMV 35S启动子,构建果实特异表达HP1、HP2双基因RNA共干涉植物表达载体pBI121-TMF7-HP1HP2.通过根癌农杆菌介导转入番茄子叶,经组织培养成功获得转基因植株.半定量RT-PCR分析显示,转基因植株果实内HP1、HP2的表达量均明显低于野生型植株果实.转基因植株果实叶绿素含量比野生型明显升高,而叶片中的叶绿素含量无明显差异.该研究结果为采用基因工程的方法改善番茄果实营养品质作出了新的尝试和提出了新的思路.
Tomato HP1 and HP2 genes are negative regulators of pigment accumulation,and they play an important role in photomorphogenesis and pigmentation regulation.In this study,a RNA interference（RNAi） vector composing of inversely-repeated sequence fragments derived from both tomato HP1 and HP2 was constructed.The CaMV 35S promoter in pBI121 was replaced by fruit-specific promoter TFM7.The construct was introduced into tomato Lysopersicon esculentum Mill.cv.Ailsa Craig by means of Agrobacterium-mediated transformation. The analysis of semi-quantitative RT-PCR revealed a distinct reduction of endogenous HP1 and HP2 expression levels in the RNAi transgenic lines compared to that of wild plants. The chlorophyll content in green fruit of the RNAi repression lines was significantly elevated, whereas the chlorophyll in their leaves remained unaltered compared with that of wild plants. The results show a new way to modify fruit nutritional quality in tomato through gene engineering. Fig 6, Tab 2, Ref 15
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2009年第5期591-595,共5页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金项目(No.90717110)
国家高技术研究发展计划("863"计划
No.2007AA10Z122)
国家杰出青年基金(No.30825030)资助~~
