摘要
从生产乙羧氟草醚工厂的污水处理池污泥中分离到一株乙羧氟草醚降解细菌,命名为YF1.根据表型特征、生理生化特性和16S rDNA序列系统发育分析,将其鉴定为假单胞菌属(Pseudomonas sp.).接种量为5%时,菌株YF1在含200mg/L乙羧氟草醚的基础盐液体培养基中降解乙羧氟草醚,7d后降解率约80%.加大接种量和外加营养碳氮源可以促进乙羧氟草醚的降解.该菌株降解乙羧氟草醚的最适pH为7.0,最适温度为30℃.菌株YF1能利用苯酚、邻苯二酚、对苯二酚、苯甲酸、龙胆酸、对硝基苯酚和邻氯苯酚为底物生长,不能利用3-苯氧基苯甲酸为碳源生长,菌株YF1细胞内邻苯二酚1,2-双加氧酶受到乙羧氟草醚或其代谢产物的诱导.
The use of organofluorine compounds has been increased in recent years, and some of the compouds are now ubiquitous environmental contaminants. Although generally viewed as recalcitrant because of their lack of chemical reactivity, a lot of fluorinated organisms are biologically active. The pesticides containing fluorine is now the hot spot of research and development. Fluoroglycofen-ethyl is one kind of herbicides having been widely used recently. A bacterial strain designated as strain YF1, capable of degrading fluoroglycofen-ethyl efficiently, was isolated from sludge in a wastewater treatment installation that had long been used to treat fluoroglycofen-ethyl polluted water. Strain YF1 was identified preliminarily as Pseudomonas sp. based on its physiological and biochemical characters and the result of the 16S rDNA homologue sequence analysis. This bacterium could degrade 80% of 200 mg/L fluoroglycofen-ethyl in 7 d. Large initial inoculum size and additional nutrients could promote the degradation. The optimal pH and temperature for fluoroglycofen-ethyl degradation were 7.0 and 30℃, respectively. Strain YFI used phenol, catechol, hydroquinol, benzoic acid, gentisic acid, p-nitrophenol and o-chlorophenol, but not 3-phenoxybenzoate as sole carbon sources. Catechol 1,2-dioxygenase, but not catechol 2,3-dioxygenase and gentisic acid 1,2-dioxygenase, was induced by fluoroglycofen-ethyl in strain YF1 cells. Fig 5, Tab 1, Ref25
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2009年第5期686-691,共6页
Chinese Journal of Applied and Environmental Biology
基金
国家高技术研究发展计划(863计划
No.2006AA10Z402)
国家自然科技平台项目~~
关键词
乙羧氟草醚
假单胞菌
生物降解
降解特性
Fluoroglycofen-ethyl
Pseudomonas
biodegradation
degradation characteristics
