摘要
目的探讨缺血缺氧脑损伤大鼠神经元特异性烯醇化酶(NSE)和S-100β蛋白(S-100β)的变化及其临床意义。方法75只Wistar大鼠随机分为对照组和实验组,再将实验组按造模后4 h、8 h、12 h和24 h不同时点分为4个亚组。利用夹闭双侧颈总动脉,并进行缺氧的方法制备模型。以放射免疫法(RIA)和酶联免疫吸附试验(ELISA)分别测定血清和脑组织中NSE和S-100β蛋白的变化。结果造模后24 h内,血清和脑组织中NSE和S-100β的含量是一个动态变化过程,呈同步变化的趋势,在12 h时均达到峰值。而且,实验组中各亚组的二者含量明显高于对照组(P<0.01或P<0.05)。结论缺血缺氧后不同时点NSE和S-100β的含量呈动态变化;二者可作为缺血缺氧后脑组织损伤程度及修复的一个临床参考指标。
Objective To explore the change and clinical significance of Neuron-specific Enolase （NSE） and S-100β protein in hypoxia-ischemia（HI） brain injured rats. Methods 75 Wistar rats were classified as control and experimental groups in random. Then the experimental group was also divided into 4 sub-groups （4 h,8 h,12 h and 24 h） after making model at the different time point. The HI model was built by occluding dipl-side common carotid artery and enforcing hypoxia. The NSE and S- 10013 concentrations were respectively determinated by radioimmunity assay （RIA） and enzyme linked immunosorbent assay （ELISA）. Results The concentrations of NSE and S-100β were dynamic change during 24 h in blood serum and brain tissue, while the change was synchronous. The both were peak value at 12 h and obviously higher in each experimental group than that in control （ P 〈 0.01 or P 〈 0.05）. Conclusions The concentrations of NSE and S-100β were synchronous change, the both were regarded as an important clinical index of HI brain injury degree and repair.
出处
《中国比较医学杂志》
CAS
2009年第9期34-37,F0002,共5页
Chinese Journal of Comparative Medicine
基金
甘肃省自然科学基金资助项目(0804NKCA105)
