摘要
目的研究地塞米松治疗放射性脑水肿与erb—B1基因转录水平的关系。方法60只大鼠建立脑胶质瘤模型后按照随机数字表法分为3组：A组（种植C6细胞15d后给予Co^60照射并于照射前后给予地塞米松治疗）、B组（种植C6细胞15d后仅给予Co^60照射）、C组（种植C6细胞后既不给予照射也不给予地塞米松治疗1．各组均为20只。所有大鼠种植C6细胞16d后将大鼠处死，测量大鼠脑水肿情况和erb—BI转录水平。结果种植C6细胞21d后3组大鼠脑水肿情况：B组脑水肿情况最严重，其次为A组、C组，任两组比较差异均有统计学意义（P〈0．05）。erb—BI转录水平：B组表达水平最高，其次为A组、C组，任两组比较差异均有统计学意义（P〈0．05）。结论地塞米松可以有效防治放射性脑水肿。地塞米松可以通过抑制erb—B1基因转录水平来治疗放射性脑水肿。
Objective To investigate the relation between the transcription level of erb-B1 gene and the therapeutic effect of dexamethasone on radiation-induced brain edema in rats. Methods Sixty rats bearing implanted C6 cell glioma in the brain were randomized into groups A, B, and C （n=20）. Fifteen days after the intracerebral cell implantation, the rats in group A received ^60Co exposure with dexamethasone treatment before and after the exposure, and those in group B had the exposure only. The rats in group C received no exposure or dexamethasone treatment after the cell implantation. Six days after ^60Co exposure, the rats were sacrificed to evaluate the brain edema and detect the expression level of erb-B 1 mRNA in the brain tissue. Results Twenty-one days after C6 cell implantation, the rats in group B showed the most serious cerebral edema, followed by those in group A and group C, with significant difference between any of the two groups （P〈0.05）. The expression level oferb-B1 mRNA differed significantly between the 3 groups, which was the highest in group B followed by group A and group C （P〈0.05）. Conclusion Dexamethasone can effectively prevent and ameliorate radiation-induced brain edema probably by suppressing the transcription level oferb-B1 gene.
出处
《中华神经医学杂志》
CAS
CSCD
北大核心
2009年第10期1024-1027,共4页
Chinese Journal of Neuromedicine
