摘要
目的:探讨应用褪黑素拮抗剂luzindole建立双足大鼠脊柱侧凸模型的可行性。方法:健康刚断乳SD大鼠30只,随机分为A、B、C、D、E共5组,每组6只。A、B、C、D四组通过截去双前肢和尾巴建立双足大鼠模型,A、B、C三组每天18:00~20:00点间腹腔注射褪黑素拮抗剂luzindole,剂量分别为0.2mg/kg、0.5mg/kg、1mg/kg;D组每天相同时间腹腔注射同体积的生理盐水;E组作为空白对照组。观察3个月,每只大鼠每天称重,每间隔1个月摄脊柱全长正位X线片,观察各组大鼠体重变化及侧凸发生情况。结果:A、B、C三组共有7只大鼠发生了脊柱侧凸,发生率为38.9%,其中A组有3只发生了脊柱侧凸,Cobb角为29°~34°,较B、C两组显著(P<0.05);D、E两组均未发生脊柱侧凸。不同时间点5组大鼠体重均有增加,体重增加最多的是注射褪黑素拮抗剂剂量最大的C组。结论:对双足大鼠使用0.2mg/kg褪黑素拮抗剂luzindole能成功建立脊柱侧凸模型;并且可促进大鼠的生长。
Objective:To discuss the feasibility and effect of establishing a scoliosis model of bipedal rats by using the antagon of melatonin receptor.luzindole.Method:Thirty healthy and weaning SD rats were divided to five groups randomly with each group of six rats.The animals in groups A, B, C and D were made for bipedal models.The group A,B and C were abdominally given the antagon of melatonin receptor,lnzindole (ip) from 6pro to 8pm every evening.The doses of luzindole were 0.2mg/kg,0.5mg/kg and lmg/kg for three groups respectively.The group D was administrated the same volume of normal saline (ip) contemporarily.The group E made up of quadruped rats were looked as sham.Three months later,all rats were weighted every day and taken X-ray of whole spine every month to note the status of scoliosis.Result:7(38.9%) animals in group A, B and C developed scoliosis.Three rats in group A had developed scoliosis with the curve Cobb's angle range of 29°-34°,which was more distinctive than those in group B and C(P〈0.05),while animals in groups D and E had no scolisis.The animals in group C accepting the highest dose of antagon of melatonin receptor had body weight increasesd most.Conclusion:Use of 0.2mg/kg antagon of melatonin (luzindole) can establish the scoliosis model successfully,and promote the growth of rats as well.
出处
《中国脊柱脊髓杂志》
CAS
CSCD
北大核心
2009年第10期774-777,共4页
Chinese Journal of Spine and Spinal Cord