摘要
为筛选新型害虫控制剂,以9种苯胺类席夫碱化合物为效应物,研究了其对菜青虫酚氧化酶活力的影响,并通过浸渍法进行活体测定。结果表明:所试9种化合物对菜青虫酚氧化酶均有一定的抑制活性,以2-羟基-4-甲氧基苯甲醛缩间氯苯胺、2-羟基-3-甲氧基苯甲醛缩苯胺、2-羟基-3-甲氧基苯甲醛缩3,4-二氯苯胺3种化合物抑制效果较好,其抑制中浓度(IC50)分别为0.051 mmol/L、0.013 mmol/L和0.059 mmol/L。动力学研究结果表明,这3种化合物对酚氧化酶的抑制作用均表现为可逆竞争性抑制。室内活体生测结果表明,这3种化合物对小菜蛾直接"杀死"活性均不高。
To screen the insecticide with novel modes of action, the inhibitory effects of 9 aniline schiff analogues as inhibitors on PO from Pieris rapae was studied by using microtitration assay, then a bio-assay was conducted by the immersion method in lab. The results showed that the tested compounds 2-hydroxy-4-methoxybenzylidene-3-chloroaniline, 2-hydroxy-3-methoxybenzylidene-anitine,2-hydroxy-3-methoxybenzylidene-3,4-diehloroaniline could inhibit PO activity obviously and the inhibitory concentration（ IC50 ） was estimated to be 0. 051 mmol/L,0. 013 mmoL/L and 0. 059 mmol/L, respectively. The inhibitors were all found to be reversible competitive inhibitors. Bio-assay of the 3 inhibitors for controlling Plutella xylostella was carried out and the results showed that the ＂killer＂ effects on Plutella xylostella was not obvious.
出处
《江苏农业学报》
CSCD
北大核心
2009年第5期1010-1014,共5页
Jiangsu Journal of Agricultural Sciences
基金
国家自然科学基金项目(30571237)
