摘要
采用ISSR分子标记,对地黄(Rehmannia glutinosa)14个自然居群、1个栽培居群和属内近缘种5个居群的遗传多样性与遗传结构进行了研究.结果表明:地黄不同居群的平均多态位点百分率(PPB)为40.53%,平均Shannon多样性指数为0.1915,平均Nei’s基因多样性指数h为0.1253,遗传多样性水平较低.AMOVA分析结果和基因分化系数显示遗传变异主要发生在居群内,居群间的遗传分化显著,其遗传分化水平介于异交物种和自交物种之间.地黄居群间的基因交流程度有限(基因流为0.3784).Mantel检测显示自然居群间遗传距离与地理距离之间没有明显相关性.推断营养繁殖占优势和受人类栽培活动的长期影响可能是导致地黄遗传多样性水平低、居群间遗传分化显著的主要原因.
In order to obtain the essential information of the genetic diversity of Rehmannia glutinosa and find the area where wild R. glutinosa exists, we investigated patterns of genetic diversity,within and among 14 natural and 1 cultural populations,in addition to 5 populations of closely related species of Rehmannia, using inter-simple sequence repeat （ISSR） DNA markers. The results showed that a relatively low genetic diversity existed in R. glulutinosa, with the average percentage of polymorphic loci 40.53 %. Moreover, most variation of R. glutinosa was found within rather than among populations as be estimated by AMOVA analysis （ФST=0.426）. The gene differentiation index Gsr（0.397 8）also showed the same situation. The gene flow was restricted among populations based on the gene flow index （Nm=0.3784）. Mantel test indicated no relationship between differentiation and geographical distance in R. glutinosa. These results interpreted that life history patterns and long cultural procession might have played critical roles in the genetic structure construction of R. glutinosa.
出处
《河南科学》
2009年第11期1386-1391,共6页
Henan Science
关键词
地黄
地黄属
遗传多样性
遗传结构
野生地黄
ISSR
Rehmanniaglutinosa
Rehmannia
genetic diversity
genetic structure
wild chinesefoxglove
ISSR
