摘要
从喜树(Camptotheca acuminate Decne)树皮中分离、纯化得到6株内生真菌,经摇瓶培养后,采用TLC法与HPLC法对其菌丝体提取物进行分析,结果表明其中1株内生真菌(XK002)产10-羟基喜树碱(10-hydroxycam ptothecin,HCPT),将此菌株命名为XK002,其10-羟基喜树碱产量为410μg/L.对菌株XK002菌落、菌丝体和孢子的有无进行了研究,表明菌株XK002属于无孢菌群(Mycelia sterlia).
Six endophytic fungi isolated from Camptotheca acuminate Decne were cultured in taper flask under shaking. The organic extracts of mycelium were analyzed with thin layer chromatography （TLC） and high performance liquid chromatography （HPLC）. The results showed one of the strains produced the 10 hydroxycamptothecin （HCPT） with output of 410 μg/L and was named XK002. Morphological observation indicates that XK002 belongs to., Mycelia sterlia.
出处
《华东师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第5期94-99,共6页
Journal of East China Normal University(Natural Science)
关键词
喜树内生真菌
分离
鉴定
10-羟基喜树碱
endophytie fungi of Camptotheca acuminate Decne
isolation, identification
10-hydroxycamptothecin
