摘要
采用杯碟法测定绿粘帚霉(RCEF4099)菌株的发酵液对8种植物病原真菌的抗菌活性。结果表明RCEF4099菌株发酵液对5种供试病原真菌的抑菌圈直径在18mm以上。对菌株发酵液的稳定性测定结果表明菌株转接6代之前,活性稳定,从第7代开始其活性缓慢降低,第10代的抑菌圈直径仅比出发菌株减少了1.7mm。RCEF4099菌株发酵液有较好的热稳定性,发酵液加热到60℃仍有较高活性。该菌株的发酵液对酸的稳定性较好,抑菌活性最强的pH值为4。
By means of cup-dish method, the anti-fungi activities of strain RCEF4099 were studied. The results showed that the antagonistic diameter of RCEF4099 to five pathogenic fungi reached more than 18mm. The stability of RCEF4099 was steady after subcultured for 6 generations. However, when subcultured from 7th generation to the 10th generation, the stability slowly decreased, which inhibitory diameter of the 10th generation decreased only 1.7mm compared to that of original strain. The fermented broth of RCEF4099 had thermal stability, it had a high anti-fungi activity to Botrytis cinerea when heated at 60℃. And it had acid and alkali stability, the highest inhibitory activity was at pH 4.
出处
《生物学杂志》
CAS
CSCD
2009年第5期22-23,29,共3页
Journal of Biology
关键词
绿粘帚霉
发酵液
抗真菌活性
稳定性
Gliocladium virens
fermentation condition
anti-fungi activities
stability
