摘要
采用MUG单管定量检测法对实验室制备的菌悬液、天然海水、水产品进行定量检测,并与大肠埃希氏菌多管发酵法的检测结果进行比对分析.结果表明:MUG单管定量检测法与大肠埃希氏菌多管发酵法对菌悬液、天然海水、水产品的检测结果没有统计学差异;MUG单管定量检测法检测数据的精确性高于大肠埃希氏菌多管发酵法,而且更为快速、经济.因此,该法适用于海水和水产品中大肠埃希氏菌的定量检测.
MUG single tube quantitative assay method was introduced to Escherichia coil assay for bacterial suspensions, natural seawater, and fishery product samples. The detected data was analyzed and compared with multiple tube fermentation method. The results showed： no significant difference was found between the results of single tube quantitative assay method and multiple tube fermentation technique; single tube quantitative assay method showed better accurateness, precision, and reproducibility than multiple tube fermentation technique; single tube quantitative assay method was quicker and more economical. The results indicated that MUG single tube quantitative assay method was suitable for the E-coli enumeration in seawater and fishery products.
出处
《集美大学学报（自然科学版）》
CAS
2009年第4期339-344,共6页
Journal of Jimei University：Natural Science
基金
国家863计划项目(2007AA09Z115)
厦门市科技计划项目(3502Z20073019)
厦门市海洋与渔业局科技项目(200707)
关键词
海水
水产品
大肠埃希氏菌
MUG单管定量检测法
大肠埃希氏菌多管发酵法
seawater
fishery product
Escherichia coli
MUG single tube quantitative assay method
multiple tube fermentation technique for Escherichia coli
