摘要
目的观察硫化氢（H2S）对脂多糖（LPS）所致大鼠内毒素性急性肺损伤（ALI）炎性细胞因子的影响，探讨其对肺脏的作用机制。方法雄性SD大鼠共48只，随机分为六组，每组8只：空白对照组、LPS组、LPS＋NaHS低剂量组、LPS＋NaHS中剂量组、LPS＋NaHS高剂量组及LPS＋PPG组。所有大鼠均检测血清中白细胞介素-1β（IL-1β）和IL-10浓度的变化，取肺组织检测肺组织中IL-1β、IL-10、黏附分子-1（ICAM-1）mRNA基因表达变化及肺组织NF-κB p65活性变化。结果与空白对照组比较，LPS组大鼠血清中IL-1β和IL-10浓度，肺组织中IL-1β、IL-10和ICAM-1 mRNA基因表达和NF-κB p65活性均明显升高（P〈0.01）。与LPS组比较，LPS＋NaHS低、中、高剂量组血清中IL-1β浓度、肺组织中IL-1β mRNA基因表达和NF-κB p65活性均明显降低；LPS＋NaHS中、高剂量组血清中IL-10浓度和IL-10 mRNA基因表达明显升高，ICAM-1 mRNA基因表达明显降低（P〈0.05或P〈0.01）。与LPS组比较，LPS＋PPG组血清中IL-1β浓度、肺组织中IL-1β和ICAM-1 mRNA基因表达及NF-κB p65活性均明显升高，血清中IL-10浓度和肺组织中IL-10 mRNA基因表达明显降低（P〈0.05或P〈0.01）。结论H2S对内毒素诱导的ALI大鼠有保护性作用，其作用机制可能与H2S调节炎性细胞因子的生成有关。
Objective To study the effect of hydrogen sulfide （H2S） on inflammatory factor in acute lung injury induced by lipopolysaccharide（LPS） in rats and to explore the mechanism. Methods Fourty - eight male rats were randomly divided into six groups （n=8）： control group, LPS group, LPS ＋ NaHS Low dose group, LPS ＋ NaHS Middle dose group, LPS ＋ NaHS High dose group, and LPS ＋ PPG group. The contents of interleukin-1β（IL-1β） and interleukin-10（IL-10）in serum, and their mRNA expression in lung tissue were respectively measured. The expression of intercellular adhesion molecule-1（ICAM-1） mRNA and the nuclear factor-κB p65（NF-κB p65） activity in lung tissue were respectively detected. Results Compared with control group, the contents of IL-1β and IL-10 in serum, the expression of IL-1β, IL-10 and ICAM-1 mRNA, and the activity of NF-κB p65 in lung tissue were significantly increased in LPS group. Compared with LPS group, the content of IL- 1β in serum, the expression of IL-1 β mRNA and the activity of NF-κB p65 in lung tissue were significantly decreased in LPS ＋ NaHS Low, Middle and High dose groups. The expression of ICAM-1 mRNA was significantly decreased, the content of IL- 10 in serum and the expression of IL- 10 mRNA were significantly increased in LPS ＋ NaHS Middle and High dose groups. Compared with LPS group, the content of IL - 1 β in serum, the expression of IL-1β and ICAM - 1 mRNA, and the activity of NF-κB p65 in lung tissue were significantly increased, while the content of IL - 10 in serum and the expression of IL - 10 mRNA were significantly decreased in the LPS ＋ PPG group. Conclusion H2S could ameliorate the ALI induced by LPS in rats, the mechanism may be H2S could regulate the inflam-matory factor＇s formation.
出处
《中国急救医学》
CAS
CSCD
北大核心
2009年第11期973-977,共5页
Chinese Journal of Critical Care Medicine
基金
河北省卫生厅重点科研课题（No.07119）.
