摘要
用ＲＡＰＤ技术鉴别了荸荠属中亲缘关系相近的两个种：Ｅｄｕｌｃｉｓ和Ｅｓｐｈａｃｅｌａｔａ。根据３个１０碱基随机引物（ＳＣ１０２３，ＳＣ１０２５和ＡＢ０１１８）获得的遗传距离值，用ＵＰＧＭＡ聚类方法构建了来自８个样点的样本的分子系统树。在分子系统树中，这８个样点的样本被分为两组：Ｅｄｕｌｃｉｓ和Ｅｓｐｈａｃｅｌａｔａ。
Random amplified polymorphic DNA (RAPD) was used to identify two closely related species of Eleocharis:E dulcis and E sphacelata. The use of three decamer primers (SC10-23, SC10-25 and AB01-18) clustered samples from 8 sites into two distinct groups: E dulcis and E sphacelata in a phylogenetic dendrogram obtained by unweighted pair group method analysis (UPGMA) of the genetic dissimilarity. RAPD assay proved to be very powerful for distinguishing these two closely related species.
出处
《湖北农学院学报》
1998年第3期213-217,共5页
Journal of Hubei Agricultural College
