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Leptin对人成熟脂肪细胞中NYGGF4基因表达的调控 被引量：2

Regulative role of leptin on NYGGF4 gene expression in matured human adipocytes
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摘要 目的:观察leptin对人成熟脂肪细胞中NYGGF4基因表达的调节作用。方法:体外培养人前体脂肪细胞,在诱导人前体脂肪细胞分化成熟的基础上,应用100ng/ml人重组leptin分别干预成熟脂肪细胞6、24h,采用实时荧光定量RT-PCR技术检测成熟脂肪细胞中NYGGF4基因mRNA的表达水平。结果:100ng/ml leptin干预人成熟脂肪细胞6h后,NYGGF4基因mRNA的相对表达量为0.000476±0.000178,显著低于对照组0.00114±0.000275,P<0.05;干预24h NYGGF4基因mRNA的相对表达量为0.000835±0.000211,也显著低于对照组0.001419±0.000193,P<0.05。结论:leptin能显著下调成熟脂肪细胞中NYGGF4基因的表达。 Objective：To explore the regulative role of leptin on NYGGF4 gene expression in matured human adipocytes. Methods： Human preadipocytes were cultured in vitro and differentiated into matured adipocytes. The matured adipocytes were treated with 100 ng/ml leptin for 6 hours and 24 hours, respectively. Total RNA from these adipocytes was extracted and the levels of N3fGGF4 mRNA expression were evaluated by real-time quantitative RT-PCR. Results：Treatment with 100 ng/ml leptin for 6 hours or 24 hours significantly decreased NYGGF4 gene mRNA expression in human matured adipocytes （P 〈 0.05）. Condusion：Leptin could downregulate the expression of NYGGF4 gene mRNA in human matured adipocytes.
作者 朱金改 赵亚萍 张春梅 陈小慧 高春林 朱春 郭锡熔
机构地区 南京医科大学附属南京妇幼保健院儿科 中国人民解放军第 南京医科大学儿科医学研究所
出处 《南京医科大学学报（自然科学版）》 CAS CSCD 北大核心 2009年第11期1477-1480,共4页 Journal of Nanjing Medical University(Natural Sciences)
基金 国家自然科学基金资助(30571978 30973231) 江苏省医学领军人才培养经费(LJ200624)
关键词 人前体脂肪细胞 诱导分化 NYGGF4基因 LEPTIN human preadipocyte cell differentiation NYGGF4 gene leptin
分类号 R329.26 [医药卫生—人体解剖和组织胚胎学]
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共引文献84

同被引文献29

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南京医科大学学报（自然科学版）
2009年 第11期

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