摘要
从土壤中分离到一株产纤维素酶的巨大芽孢杆菌AP25,经羧甲基纤维素平板检测,该菌可产生葡聚糖内切酶。根据GenBank登录的-β1,4内切葡聚糖酶基因(DQ782954.1,M28332.1,AY859492.1)的同源性序列,利用PCR方法克隆到该酶的基因,并对其进行测序。测序结果显示其全长为1500bp,推测其含499个氨基酸。与GenBank中的已知葡聚糖内切酶相比较,发现该酶的氨基酸序列与Bacillus subtilis的β-1,4内切葡聚糖酶基因同源性达到达94%。
Bacillus. megaterium AP25 screened from Australia wet soil could produce endo-β- glucanase that was detected on CMC-Na plates. We amplified the endo-β-1 ,4-glucanase gene sequenced it according to GenBank No. DQ782954.1, M28332.1, AY859492.1. Its full length 1500bp and its amino acid number was about 499. Its amino acid sequence had 98% homology that of the endo-β-1 ,4-glucanase gene of Bacillus subtilis.
出处
《山东科学》
CAS
2009年第5期22-26,共5页
Shandong Science
基金
山东省科技攻关项目(2007GG10005005)
