摘要
目的:探讨乙酰胆碱酯酶(acetylcholinesterase,AChE)在不同种类和不同品系蚊虫间的差异,为应用生物化学方法检测蚊虫的抗药性提供依据。方法:以碘化硫代乙酰胆碱为底物,5,5′-二硫-双(2-硝基苯甲酸)为显色剂,测定单个蚊虫AChE,以残杀威为抑制剂测定AChE不敏感性。结果:4种类敏感蚊虫AChE活性,龄幼虫间和3日龄雌成虫间均有非常显著性差异(P<0.01),而且3日龄雌成虫的AChE活性均明显高于其龄幼虫(P<0.01)。抗敌敌畏(Rd)、抗残杀威(Rp)品系淡色库蚊AChE活性水平高于敏感(S)品系(P<0.01),抗氯氰菊酯(Rc)品系淡色库蚊AChE活性与S品系相近(P>0.05)。Rd、Rp品系淡色库蚊不敏感AChE个体频率高于S品系(P<0.01),Rc品系淡色库蚊不敏感AChE个体频率与S品系相近(P>0.05)。结论:测定AChE可用来判断蚊虫抗药性状况,而测定对象宜选择3日龄雌成虫。
Objective： To investigate the diversity of acetylcholinesterase （ACHE） activity in variety classes and strains of Culex pipiens pallens and provide resistance detection of mosquito by biochemical method. Methods： a basis for the insecticide- AChE insensitivity of single mosquito was determined, using acetythiocholine iodide （ATch） as the substrate, 5,5＇-dithio-b S （2-nitrobenzoic acid） （DTNB） as the developer,and propoxur as the inhibitor. Results： There were significant differences in AChE activity among the four types of Ⅳ instar larvae and 3-day- old adult female of sensitive strain mosquito（P〈0.01）. The AChE activity of the 3-day-old adult female was higher than that of Ⅳ instar larvae of the four types of sensitive strain mosquito（P〈 0. 01）. The AChE activity of anti-DDVP （Rd） and anti-propoxur （Rp） strains of Culex pipiens pallens was significantly higher than that of sensitive （S） strain （P〈0.01）, while the AChE activity of anti-cypermethrin （Rc） strain of Culex pipiens pallens was similar to that of S strain （P〉0. 05）. The individual frequency of insensitive AChE of Rd and Rp strains of Culex pipiens pallens was significantly higher than that of sensitive （S） strain（P〈0. 01） ,while the individual frequency of insensitive AChE of Rc strain of Culex pipiens pallens was similar to that of S strain （P〉0.05）. Conclusion： The AChE activity determination can be used to examine the insecticide- resistance of mosquito.
出处
《浙江大学学报（医学版）》
CAS
CSCD
北大核心
2009年第5期511-514,共4页
Journal of Zhejiang University(Medical Sciences)
基金
山东省卫生厅(2001CA2CEB1)
济宁市科技局资助项目
