期刊文献+

Specifically Binding of L-ficolin to N-glycans of HCV Envelope Glycoproteins E1 and E2 Leads to Complement Activation 被引量:8

Specifically Binding of L-ficolin to N-glycans of HCV Envelope Glycoproteins E1 and E2 Leads to Complement Activation
原文传递
导出
摘要 L-ficolin, one of lectin families, is a recently identified complement factor that initiates lectin pathway of complement. Little is known about its role in viral hepatitis. In the present study, we found that L-ficolin in serum from 103 patients with hepatitis C virus (HCV), were significantly higher than that in 150 healthy controls. We further found that L-ficolin expressions were significantly increased in vitro study by HCV JFH-1 infected human hepatocyte cell line Huh7.5.1. Investigation of the mechanisms of the L-ficolin action on HCV demonstrated that L-ficolin protein could recognize and bind to envelope glycoproteins E1 and E2 of HCV, activating the lectin complement pathway-mediated cytolytic activity in HCV-infected hepatocyte. This interaction between L-ficolin and HCV E1 and E2 glycoproteins was attributed to the N-glycans of E1 and E2. These findings provide new insights into the biological functions of L-ficolin in clinically important hepatic viral diseases. L-ficolin, one of lectin families, is a recently identified complement factor that initiates lectin pathway of complement. Little is known about its role in viral hepatitis. In the present study, we found that L-ficolin in serum from 103 patients with hepatitis C virus (HCV), were significantly higher than that in 150 healthy controls. We further found that L-ficolin expressions were significantly increased in vitro study by HCV JFH-1 infected human hepatocyte cell line Huh7.5.1. Investigation of the mechanisms of the L-ficolin action on HCV demonstrated that L-ficolin protein could recognize and bind to envelope glycoproteins E1 and E2 of HCV, activating the lectin complement pathway-mediated cytolytic activity in HCV-infected hepatocyte. This interaction between L-ficolin and HCV E1 and E2 glycoproteins was attributed to the N-glycans of E1 and E2. These findings provide new insights into the biological functions of L-ficolin in clinically important hepatic viral diseases.
出处 《Cellular & Molecular Immunology》 SCIE CAS CSCD 2009年第4期235-244,共10页 中国免疫学杂志(英文版)
基金 Acknowledgments This work was supported by grants to X.-L. Zhang from the National Natural Science Foundation of China (30870122, 30670098 and 20532020), 973 Program of China 2006CB 504300 and 2009CB522507, National Grand Program on Key Infectious Disease (2008ZX10003-005), Hubei Province Science Technology Department (2006ABD007, 2007ABC 010) and Hubei Ministry of Public Health of (JX1B074).
关键词 L-FICOLIN hepatitis C virus envelope glycoproteins COMPLEMENT viral hepatitis L-ficolin, hepatitis C virus, envelope glycoproteins, complement, viral hepatitis
  • 相关文献

参考文献37

  • 1Poynard T, Yuen MF, Ratziu V, Lai CL, Viral hepatitis C. Lancet. 2003;362:2095-2100.
  • 2Sarasin-Filipowicz M, Oakeley E J, Duong FH, et al. Interferon signaling and treatment outcome in chronic hepatitis C. Proc Natl Acad Sci U S A. 2008,105:7034-7039.
  • 3Zhong J, Gastaminza P, Cheng G, et al, Robust hepatitis C virus infection in vitro. Proc Natl Acad Sci U S A. 2005;102:9294- 9299.
  • 4Wakita T, Pietschmann T, Kato T, et al Production of infectious hepatitis C virus in tissue culture from a cloned Viral Genome.Nat Med. 2005; 11:791-796.
  • 5Lindenbach BD, Evans MJ, Syder AJ, et al. Complete replication of hepatitis C virus in cell culture. Science. 2005; 309:623-626.
  • 6Goffard A, Callens N, Bartosch B, et al. Role of N-linked glycans in the functions of hepatitis C virus envelope glycoproteins. J Virol. 2005;79:8400-8409.
  • 7Beyene A, Basu AK, Mayer RR. Influence of N-linked glycans on intracellular transport of hepatitis C virus E1 chimeric glycoprotein and its role in pseudotype virus infectivity. Virology. 2004;324:273-285.
  • 8Flint M, McKeating JA. The role of the hepatitis C virus glycoproteins in infection. Rev Med Virol. 2000; 10:101-117.
  • 9Fujita T. Evolution of the lectin-complement pathway and its role in innate immunity. Nat Rev Immunol. 2002;2:346-353.
  • 10Matsushita M, Fujita T. Ficolins and the lectin complement pathway. Immunol Rev. 2001;180:78-85.

同被引文献4

引证文献8

二级引证文献12

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部