摘要
目的探讨人参皂甙Rg3对人肺鳞癌SK-MES-1细胞的诱凋亡作用及其对凋亡相关基因survivin的影响。方法人肺鳞癌SK-MES-1细胞分为Rg3实验组、空白对照组和阳性对照组,阳性对照药物为三氧化二砷。流式细胞仪检测各组细胞的凋亡率;免疫细胞化学法和RT-PCR法检测survivin蛋白和mRNA的表达。结果不同浓度的人参皂甙Rg3处理SK-MES-1细胞48h后,细胞凋亡率增加,作用呈剂量依赖关系。人参皂甙Rg3能够下调survivin蛋白和mRNA的表达,各实验组与空白对照组间比较有统计学意义(P<0.05)。结论人参皂甙Rg3对人肺鳞癌SK-MES-1细胞具有诱导凋亡的作用,其机制可能与其抑制survivin基因的表达有关。
Objective To investigate the effect of ginsenoside Rg3 on the apoptosis and survivin expression in human lung squamous cell carcinoma cell line SK-MES-1. Methods SK-MES-1 cells were divided into Rg3 treatment group, blank control group and positive control （arsenic trioxide） group. The apoptotic rate of the cells in each group was determined using flow cytometry, and the expression of survivin protein and mRNA was detected by immunocytochemistry and RT-PCR, respectively. Results A 48-h treatment with Ginsenoside Rg3 induced increased apoptotic rate of SK-MES-1 cells in a dose-dependent manner. Ginsenoside Rg3 significantly downregulated the expressions of survivin protein and mRNA as compared with the expression levels in the blank control group （P〈0.05）. Conclusion Ginsenoside Rg3 can induce the apoptosis of SK-MES-1 cells, the mechanism of which may involve inhibited survivin expression.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2009年第9期1823-1826,共4页
Journal of Southern Medical University
基金
河南省教育厅自然科学研究资助计划项目(2009A320046)
