摘要
目的:探讨EphA4在海人酸诱导大鼠大脑皮层神经元凋亡过程中的表达变化,及天麻素对EphA4表达的影响。方法:提取新生大鼠大脑皮层神经元体外培养,7d后随机分为对照组、海人酸模型组、海人酸+天麻素干预组;采用倒置相差显微镜观察神经元形态学变化,Hoechst33258荧光染色、AO/EB荧光染色、LDH活性测定检测神经元凋亡和坏死情况,CY3荧光染色检测EphA4表达变化。结果:海人酸模型组较对照组神经元凋亡率显著升高(P<0.01),EphA4表达显著增高(P<0.01);海人酸+天麻素干预组较海人酸模型组神经元凋亡率显著下降,EphA4表达上调显著受抑。结论:海人酸诱导大鼠大脑皮层神经元凋亡过程中EphA4表达增高,天麻素在这一过程中抑制EphA4表达,对神经元具有一定保护作用。
AIM： To explore the expressions of EphA4 during apoptosis in rat cerebral cortical neurons induced by kainic acid（KA） and the effect of gastrodin on expressions of EphA4. METHODS： Neurons from newborn rat cerebral cortex were cultured in vitro for seven days, and were divided into three groups at random： control group, exposure to KA group, and exposure to KA and gastrodin group. Morphologic changes of neurons were observed with inverted phase contrast microscope. Apoptosis and necrosis of neurons were detected by staining of fluorescein Hoechst 33258 or AO/EB, and measurement of LDH. Expressions of EphA4 were measured by specific antibody labeled with CY3. RESULTS： Neuronal apoptotic ratios were notably increased and expressions of EphA4 were notably up - regulated in exposure to KA group compared to control group. Neuronal apoptotic ratios were notably decreased and up - regulations of EphA4 were notably inhibited in exposure to KA and gastrodin group compared to exposure to KA group. CONCLUSION： The expression of EphA4 is increased during apoptosis in rat cerebral cortex neurons induced by KA. Gastrodin inhibits the expression of EphA4 and has a neuroprotective effect during this process.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2009年第10期1917-1921,共5页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30770758)
河南省教育厅自然科学研究计划资助项目(No.2008A320032)
