摘要
[目的]了解临床分离的耐亚胺培南铜绿假单胞菌(PAE)的相关耐药基因存在状况。[方法]对16株铜绿假单胞菌采用PCR检测亚胺培南耐药的相关基因(TEM、OXA-10群、SHV、oprD2、IMP、CARB、blaDHA、VIM、PER、GES、VEB)。[结果]16株铜绿假单胞菌TEM阳性4株(25.00%)、OXA-10群阳性5株(31.25%)、SHV阳性5株(31.25%)、orpD2阳性16株(100%)、IMP阳性2株(12.50%)CARB阳性1株(6.30%)、blaDHA阳性1株(6.30%)、VIM阳性1株(6.30%)、PER阳性0株(0)、GES阳性1株(6.30%)、VEB阳性0株(0),且部分菌株携带3个以上的耐药基因。[结论]临床分离的耐亚胺培南铜绿假单胞菌携带多种耐药基因,没有导致院内感染的流行耐药菌株。
[Objective] To investigate genes associated with the drug-resistance of Imipenem antibiotics in Pseudomonas aeruginosa（PAE） isolated from clinical patients.[Methods] Genes including TEM,SHV,OXA-10,IMP,oprD2,CARB,blaDHA,VIM,PER,GES and VEB were detected by PCR amplification in 16 PAE isolates.[Results] TEM,SHV,IMP,OXA-10,oprD2,CARB,blaDHA,VIM,PER,GES and VEB genes were positive in 25.00%,31.25%,31.25%,12.50%,100%,6.30%,6.30%,6.30%,0,6.30% and 0 of 16 isolates.Some of the strains carried more than 3 resistance genes.[Conclusion] Pseudomonas aeruginosa carries various and more than one beta-lactamase genes in clinical Patients.
出处
《大连医科大学学报》
CAS
2009年第5期583-586,共4页
Journal of Dalian Medical University
关键词
铜绿假单胞菌
亚胺培南
耐药性
基因
Pseudomonas aeruginosa
Imipenem
drug susceptibility
gene
