摘要
目的1996年5~6月在黑龙江省绥芬河、东宁地区进行斑点热病的调查。方法采用micro-IF、病原分离及PCR/RFLP等方法。结果(l)发现被蜱叮咬后斑点热临床指征的疑似病人7例,其中血清检测有4例早期与恢复期血清抗SFG立克次体lgM、IgG抗体滴度≥4倍升高;(2)从病人血液分离到1株SFG立克次体H-5株,经鉴定显示该分离株和黑龙江省立克次体54株一致,和SFG各标准株有明显差异。结论该患者为SFG黑龙江立克次体感染,从病原学角度证实该立克次体对人具有致病性。
Aim Spotted fever disease was investigated at Suifenhe and Dongning area in Heilongjiang province in May and Jun, 1996. Method Micro─IF, isolation and PCR/RFLP were used. Result (1) 7 persons bited by ticks shown spotted fever clinical manifestation. IgG and IgM aginst SFG were detected in 4 of the 7 person's serum. The titer of IgM and IgG in convalescent specimens was 4 time higher than that of -acute specimens. (2) A SFG rickettsiae strain was isolated from the patient's blood specimen firstly identification by micro ─IF cross test and PCR/RFLP show that the new isolated H─ 5 strain was indentical with R. heilongjiang. Conclusion These results indicated that the patient was infeted with SFG R. heilongjiang and SFG R. Halongpeng can cause disease in human.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1998年第6期23-26,共4页
Chinese Journal of Zoonoses