摘要
采用Tru9Ⅰ/EcoRⅠ和TaqⅠ/PstⅠ两种限制性内切酶酶切组合对细鳞鱼Brachymystax lenok pallas的遗传多样性进行研究,探索适合其AFLP的双酶切体系。每种双酶切组合体系分别选用20对选择性扩增引物进行扩增。结果表明：采用Tru9Ⅰ/EcoRⅠ组合共扩增出了739个位点,其中多态性位点为336个,每对引物组合扩增的位点为21-45,位点多态性百分比为45.47%,平均Shannon氏指数为0.2739;而采用TaqⅠ/PstⅠ组合共扩增出了759个位点,多态性位点为405个,每对引物组合扩增的位点为29-53,位点多态性百分比为53.36%,平均Shannon氏指数为0.3144。表明,对细鳞鱼进行AFLP分析时,TaqⅠ/PstⅠ的酶切体系呈现出更大的多态性,能提供更多的可供分析研究的数据,更适用于细鳞鱼基因组分析。
Two kinds of AFLP reaction system with the different restriction enzyme combinations （ Tru9 Ⅰ/Ecor Ⅰand Taq Ⅰ/Pst Ⅰ ） were compared in lenok Brachymystax lenok （Palls）. Twenty pairs of selective amplification primers were chosen for each binary enzymes cutting group. Total of 739 loci were obtained in the group of Tru 9 Ⅰ / EcoR Ⅰ , with 336 polymorphism loci, in which there were 21 to 45 loci each pair of the primers, with the polymorphism loci of 45.47%, and Shannon＇s Information index （Ⅰ） of 0.2739. In the group of Taq Ⅰ/Pst Ⅰ , however, there were 759 loci with 405 polymorphism loci, 29 to 53 loci for each pair of the primers, the polymorphism loci of 53.36%, and the Shannon＇s Information index of 0.3144, indicating that the AFLP reaction system with the Taq Ⅰ/Pst Ⅰ restriction enzymes had the greater polymorphism and offered more scientific data of the lenok. So the group of Taq Ⅰ and Pst Ⅰ I was considered the suitable restriction enzymes to the AFLP genetic diversity analysis of the fish.
出处
《大连水产学院学报》
CSCD
北大核心
2009年第5期459-464,共6页
Journal of Dalian Fisheries University
基金
国家支撑计划项目(2006BAD03B08-3)
黑龙江省科技攻关项目(GC03B511
GA06B203-4)
黑水研基本科研业务费资助项目(2007HSYZX-YY-21
2008HSYZX-YZ-03)
