摘要
目的:观察联合顺次法对实验兔膝骨关节炎软骨bFGF的表达及组织形态的影响,探讨其治疗骨关节炎疗效机理。方法:将70只日本大耳白兔随机分为正常组、造模组、关节冲洗组、透明质酸注射组、中药离子导入组、骨友灵擦剂组、联合顺次组。右侧股静脉结扎造模,造模8周后分别以相应的方法治疗,治疗5周后,切取右股骨内髁软骨,应用免疫组化法检测软骨中bFGF的表达及Wakitani法量化评定软骨组织形态变化,结果采用t检验统计分析。结果:各治疗组关节软骨bFGF表达与正常组、造模组比较有明显增高(P<0.01),其中联合顺次组最明显,与其他治疗对照组比较差异有统计学意义(P<0.05);各治疗组关节软骨组织形态评分比造模组有明显下降(P<0.01),其中联合顺次组最明显,与其他治疗对照组比较差异有统计学意义(P<0.01)。结论:联合顺次法可明显增强兔膝骨关节炎软骨bFGF的表达,从而有效地保护和促进关节软骨的修复。
Objective：To observe the influence of the combined therapy in order（CTO）on bFGF expression and tissue morphology in knee osteoarthritis（KOA）rabbits.Methods：Seventy Japanese rabbits were randomly divided into normal group,model group,joint flushing group,hyaluronic acid group,traditional Chinese medicine ion introduction group,Guyouning group and CTO group.The KOA models were established by hypodesis of right lateral femoral venous.The rabbits were treated with the above methods 8 weeks after operation.After 5 weeks＇ treatment, the right femoral condyle cartilage was collected to measure bFGF expression with immunohistochemical method. The change of tissue morphology was observed with Wakitani method. Results：Compared with control group and model group, bFGF expression was significantly increased in the rest groups （P〈0. 01）, in which bFGF was highest in CTO group （P〈0. 05）. Compared with model group, the score of tissue morphology was markedly decreased in treatment groups （P〈0.01）, in which the score was lowest in CTO group （P〈0.01）. Conclusion：CTO can obviously increase bFGF expression in cartilage of knee osteo arthritis rabbits and effectively promote cartilage repair.
出处
《中国中医骨伤科杂志》
CAS
2009年第9期1-3,共3页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
湖北省教育厅科研基金资助项目
编号:Q200716006
