摘要
目的:观察耳针对阿尔茨海默病(AD)大鼠学习记忆能力的影响,并探讨其可能机制。方法:30只SD大鼠随机分为对照组、模型组、耳针组,每组10只。模型组、耳针组采用海马CA1区微量多次注射冈田酸(OA)建立AD大鼠模型,对照组注入等体积的对照液二甲亚砜(DMSO)。耳针组采用针刺"脑""肾"耳穴治疗,模型组、对照组大鼠耳部未做处理。通过Morris水迷宫观察大鼠行为学改变,免疫组化方法观察胆碱乙酰转移酶(ChAT)和胶质纤维酸性蛋白(GFAP)的表达。结果:耳针治疗后,与模型组比较,耳针组Morris水迷宫定向航行试验中AD大鼠平均逃避潜伏期明显缩短(P<0.01),空间探索试验中撤去站台后原站台象限活动时间明显延长(P<0.05),穿越站台次数明显增多(P<0.01),海马及皮层ChAT表达增多(P<0.01,P<0.05),而海马CA1区GFAP阳性反应物表达明显减少(P<0.01)。结论:耳针可以改善AD大鼠学习记忆能力,其可能机制是耳针降低胆碱能神经元的损伤以及减少星形胶质细胞的异常活化与增生。
Objective To observe the effects of auricular acupuncture on the learning and memory abilities of model rats with Alzheimer＇s disease（AD）, and investigate its mechanism. Methods Thirty SD rats were randomly divided into a control group, a model group and an auricular acupuncture group, 10 rats in each group. The model rats with AD were established by multiple injections with Okadaic Acid into the CA1 region of hippocampus. In the control group, the same quantity injection with Dimethyl Sulfoxide （DMSO） was applied on experimental rats. The auricular acupoints of ＂Nao＂ （brain） and ＂Shen＂ （kidney） were used for treating in the auricular acupuncture group, in contrast, the auricular region were not treated in the model and the control groups. The learning and memory capabilities of the rats were assessed with Morris Water Maze behavioral test, and the expressions of choline acetyltransferase （CHAT） and glial fibrillary acidic protein （GFAP） were examined by immunohistochemistry. Results Comparing with the model group, the treated AD rats with auricular acupuncture was showed that the average es- cape latency was obviously shortened in the place navigation test （P〈0.01）, the movement time in plateform quad- rant was obviously prolonged in the spatial probe test （P〈0.05）, and the number of traversing platform obviously increased （P〈0.01） after the platform was taken away. The expression of ChAT increased in the hippocampus and cortex （P〈0.01, P〈0.05）, but the expression of GFAP obviously decreased in the CA1 region of hippocampus （P〈0. 01）. Conclusion Auricular acupuncture can improve the learning and memory capability of the model rats with AD. Its mechanism might be related with decreasing eholinergic neuron damage and reducing the abnormal activation and hyperplasia of astrocyte.
出处
《中国针灸》
CAS
CSCD
北大核心
2009年第10期827-832,共6页
Chinese Acupuncture & Moxibustion
基金
贵州省优秀科技教育人才省长专业基金资助项目:S2001-9
