摘要
目的:了解肢体制动对大鼠胰岛素敏感性的影响.方法:将SD大鼠随机分为对照组、制动组,每组20只.制动组大鼠右后肢石膏固定,对照组不做特殊处理.3wk后将两组大鼠行高胰岛素—正常葡萄糖钳夹技术,术后分离大鼠右比目鱼肌测量质量、肌细胞直径及肌纤维截面积.结果:制动组稳态葡萄糖输入速率[(35±5)mg(/kg·h)vs(13±4)mg/(kg.h)]、右侧比目鱼肌湿质量[(169.2±4.1)gvs(67.1±6.3)g]、肌细胞直径[(49.8±2.7)μmvs(20.6±3.2)μm]、肌纤维截面积[(998±21)μm2vs(579±14)μm2]均明显低于对照组.结论:局部制动可导致大鼠骨骼肌萎缩,并使机体对胰岛素的敏感性降低.
AIM：To observe the effects of limb immobilization on the insulin sensitivity.METHODS：Sprague-Dawley rats were randomly assigned to 2 groups：control（CON）and three-week immobilization（IB）.The right legs of the rats in IB group were immobile for three weeks.After the 3 weeks,hyperinsulinem ic-euglycemic clamp was used to evaluate the insulin sensitivity and soleus muscles were dissected and weighted.The diameter and cross section area of muscle cell were measured.RESULTS：In IB group,the wet weights of the soleus [（169.2±4.1）g vs（67.1±6.3）g],the diameter of the muscle cell [（49.8±2.7）μm vs（20.6±3.2）μm）] and the area of its cross section decreased [（998±21）μm2 vs（579±14）μm2）] obviously as compared with those in CON group.The glucose infusion rate was significant lower than that in CON group [（35±5）mg/（kg·h）vs（13±4）mg/（kg·h）].CONCLUSION：Limb immobilization induces soleus atrophy and decreases the insulin sensitivity.
出处
《第四军医大学学报》
北大核心
2009年第19期1898-1900,共3页
Journal of the Fourth Military Medical University
基金
四川省科技厅应用基础项目(07JY029-117)
国家体育总局运动医学重点实验室专项基金
关键词
胰岛素敏感性
高胰岛素-正糖钳夹
制动
骨骼肌萎缩
insulin sensitivite
hyperinsulinem ic-euglycem ic clamp
Immobilization
soleus atrophy
