摘要
对产朊假丝酵母进行紫外诱变获得高产油脂突变菌株。紫外诱变后筛选出产油量最多菌种。通过正交试验得到产朊假丝酵母产油脂的最优条件:培养温度25℃,培养时间4d,接菌量10%,转速100r/min,在此条件下培养,紫外诱变10s后的产朊假丝酵母产油脂量可达到18%。
Mutant Candida utilis strain with high yield of lipid was obtained by UV mutation. The optimum conditions for lipid production for mutant strain by orthogonal experiment were inoculation of 10%, culture at 25℃ with rotation at 100 r/min for 4 d. The maximum yield of lipid reached to 18% occurred at the strain treated with UV mutation for 10 s with optimum culture conditions.
出处
《中国酿造》
CAS
北大核心
2009年第11期32-33,共2页
China Brewing
关键词
酵母
筛选
紫外诱变
油脂
yeast
screening
UV mutation
lipid
