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猪流感病毒NP基因的克隆及杆状病毒转移载体的构建 被引量：3

Cloning of NP Gene of Swine Influenza Virus and Construction of Baculovirus Transfer Vector
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摘要 本研究通过RT-PCR扩增了猪流感病毒A/Swine/Inner Mongolia/547/01(H3N2)的NP基因,并将其克隆入pMD18-T载体。重组pMD18-T经SalI和EcoR I酶切后得到的NP基因插入pMelBacB载体的SalI/EcoRI位点。PCR和限制性内切酶分析鉴定表明成功构建了pMelBacB-NP转移载体。这为开发NP诊断抗原和亚单位疫苗奠定了基础。 In the study,NP gene of A/ Swine/ Inner Mongolia/ 547/ 01 （H3N2）was amplified by RT-PCR.The product of RT-PCR was cloned into pMD 18-T vector.NP gene was inserted into the Sal I/EcoR I sites of pMelBacB after the recombinant pMD18-T was cleavaged with restriction endonuclease Sal I and EcoR I.Identification by PCR and restriction-endonuclease analysis showed that the recombinant transfer vector pMelBacB-NP was constructed successfully.It was helpful for development of diagnostic antigen and subunit vaccine based on NP
作者 赵朴 郑玉姝 贾贝贝 乔传玲 陈化兰 刘兴友 李海燕
机构地区 河南科技学院动物科学学院 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/农业部动物流感重点开放实验室
出处 《西南农业学报》 CSCD 北大核心 2009年第5期1453-1455,共3页 Southwest China Journal of Agricultural Sciences
关键词 猪流感病毒 NP基因 克隆 转移载体 Swine influenza virus NP gene Cloning Transfer vector
分类号 S828 [农业科学—畜牧学]
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二级参考文献21

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共引文献12

同被引文献25

  • 1陈君彦,李海燕,申之义,陈化兰,于康震,毕英佐.H1N1亚型猪流感病毒中国分离株血凝素基因分子演化的研究[J].中国预防兽医学报,2005,27(1):13-17. 被引量：20
  • 2吴华,郭万柱.猪流感病毒分子生物学研究进展[J].中国预防兽医学报,2006,28(2):238-240. 被引量：19
  • 3王曲直,龙进学,胡顺林,吴艳涛,刘秀梵.神经氨酸酶茎部氨基酸缺失对H5N1亚型禽流感病毒生物学特性的影响[J].微生物学报,2006,46(4):542-546. 被引量：14
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引证文献3

二级引证文献11

西南农业学报
2009年 第5期

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