摘要
目的建立紫果西番莲叶提取物中异荭草素和荭草素的含量测定方法。方法采用HPLC法。Inertsil C18色谱柱(4.6mm×250mm,5μm);流动相:水-四氢呋喃-异丙醇-乙腈(87∶8∶2∶3);柱温:30℃,流速:1mL/min;检测波长:360nm。结果异荭草素、荭草素回归方程:Y=2.901×104X-188.1,r=0.999 8;Y=1.824×104X-228.83,r=0.999 5;线性范围分别为0.012～0.602、0.009～0.451mg.mL-1。平均回收率分别为100.09%、98.66%,RSD分别为2.10%、1.81%。结论HPLC法测定紫果西番莲叶提取物中异荭草素和荭草素的含量,专属性强、精密度好、操作简单、准确性高。
Aim To establish a method for detecting isorientin and orientin by HPLC. Methods The chromatographic conditions include the Intersil C18 column and a mobile phase consisting of a trinal aquatic solution , i. e. H2O-tetrahydrofuran-isopropanol-acetonitrile （87： 8： 2：3 ）. The flow rate was 1.0mL/min. The detection wave- length was 360nm. The temperature was 30℃. Results The regression equation for isorientin and orientin was：Y = 2.901 × 10^4X - 188.1, （ r = 0.999 8） ; Y = 1. 824 × 10^4X - 228.83 （ r = 0. 999 5 ） respectively ; the linear range was 0.012 -0. 602mg.mL^-1 ,0.009 - 0. 451mg.mL^-1 respectively; the average recovery （ RSD ） was 100.09% （2.10） ,98.66% （ 1.81 ） respectively. Conclusions The method is rapid ,accurate and reliable.
出处
《解放军药学学报》
CAS
2009年第5期443-445,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
基金
广州市科委基础性研究计划项目
No.2005J1-C0352
